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Biodegradation of binary mixtures of octane with benzene, toluene, ethylbenzene or xylene (BTEX): insights on the potential of Burkholderia, Pseudomonas and Cupriavidus isolates
World Journal of Microbiology and Biotechnology ( IF 4.1 ) Pub Date : 2021-06-21 , DOI: 10.1007/s11274-021-03093-4
Hernando P Bacosa 1, 2 , Jhonamie A Mabuhay-Omar 3 , Rodulf Anthony T Balisco 3 , Dawin M Omar 4 , Chihiro Inoue 2
Affiliation  

The contamination of the environment by crude oil and its by-products, mainly composed of aliphatic and aromatic hydrocarbons, is a widespread problem. Biodegradation by bacteria is one of the processes responsible for the removal of these pollutants. This study was conducted to determine the abilities of Burkholderia sp. B5, Cupriavidus sp. B1, Pseudomonas sp. T1, and another Cupriavidus sp. X5 to degrade binary mixtures of octane (representing aliphatic hydrocarbons) with benzene, toluene, ethylbenzene, or xylene (BTEX as aromatic hydrocarbons) at a final concentration of 100 ppm under aerobic conditions. These strains were isolated from an enriched bacterial consortium (Yabase or Y consortium) that prefer to degrade aromatic hydrocarbon over aliphatic hydrocarbons. We found that B5 degraded all BTEX compounds more rapidly than octane. In contrast, B1, T1 and X5 utilized more of octane over BTX compounds. B5 also preferred to use benzene over octane with varying concentrations of up to 200 mg/l. B5 possesses alkane hydroxylase (alkB) and catechol 2,3-dioxygenase (C23D) genes, which are responsible for the degradation of alkanes and aromatic hydrocarbons, respectively. This study strongly supports our notion that Burkholderia played a key role in the preferential degradation of aromatic hydrocarbons over aliphatic hydrocarbons in the previously characterized Y consortium. The preferential degradation of more toxic aromatic hydrocarbons over aliphatics is crucial in risk-based bioremediation.



中文翻译:

辛烷与苯、甲苯、乙苯或二甲苯 (BTEX) 的二元混合物的生物降解:对伯克霍尔德菌、假单胞菌和铜绿假单胞菌分离株潜力的见解

原油及其副产品(主要由脂肪烃和芳香烃组成)对环境的污染是一个普遍存在的问题。细菌的生物降解是去除这些污染物的过程之一。进行这项研究是为了确定伯克霍尔德氏菌的能力。B5, Cupriavidus sp. B1,假单胞菌属。T1 和另一个Cupriavidussp. X5 在有氧条件下以 100 ppm 的最终浓度降解辛烷(代表脂肪烃)与苯、甲苯、乙苯或二甲苯(BTEX 作为芳香烃)的二元混合物。这些菌株是从更喜欢降解芳香烃而不是脂肪烃的富集细菌聚生体(Yabase 或 Y 聚生体)中分离出来的。我们发现 B5 比辛烷更快地降解所有 BTEX 化合物。相比之下,与 BTX 化合物相比,B1、T1 和 X5 使用了更多的辛烷值。B5 也更喜欢使用苯而不是辛烷,浓度不超过 200 毫克/升。B5 具有烷烃羟化酶(alk B) 和儿茶酚 2,3-双加氧酶 (C23D) 基因,它们分别负责烷烃和芳烃的降解。这项研究有力地支持了我们的观点,即伯克霍尔德氏菌在先前表征的 Y 财团中芳烃优先降解超过脂肪烃方面发挥了关键作用。在基于风险的生物修复中,毒性更强的芳烃优先降解而不是脂肪烃。

更新日期:2021-06-21
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