Propagation of grape rootstocks with cuttings is one of the routine processes of viticulture. Cutting studies for nursery production under nursery conditions often fall below the expected productivity level due to biotic and abiotic stress. To increase nursery yield remains on the agenda of vine researchers. Encapsulated silver nanoparticles (AgNPs) were produced by the green synthesis method from grape seed extract (Vitis vinifera L. cv Öküzgözü) with high phenolic content and silver nitrate (AgNO). The size of AgNPs was determined by Transmission Electron Microscope (TEM) as 10–50 nm and was not agglomerated and browned in colour. The effect of AgNPs on rooting of standard lignified 41B (Vitis vinifera L. cv. Chasselas × Vitis berlandieri) rootstocks pure and with Indole 3 Butyric Acid (IBA) (control, 0.5 mg L⁻¹ AgNPs, 0.5 mg L⁻¹ AgNPs 50 ppm IBA, 1 mg L⁻¹ AgNPs, 1 mg L⁻¹ AgNPs 50 ppm IBA and 50 ppm IBA) were tested. The effects of the applications on the cuttings in the greenhouse 1:1 perlite peat medium, leaf chlorophyll content, vegetative growth parameters and the effects on the vascular tissues were evaluated by micro tomography (microCT). Applications of pure AgNPs and combined with IBA did not altered the leaf chlorophyll content. While 1 mg L⁻¹ AgNPs application, which was the closest level to 50 ppm IBA, provided the root and shoot development, while the application of 1 mg L⁻¹ AgNPs encouraged the development of shoot most. The 1 mg L⁻¹ AgNPs + 50 ppm IBA application resulted in the highest number of nodes in shoots developing from cuttings. In microCT analysis of rooted and unrooted grapevine cuttings, tissue damage was determined that was attributed to result in drying due to embolism and loss of nursery material. AgNPs applications are thought to increase the nursery success in grapevine rootstock cuttings.

葡萄砧木插条繁殖是葡萄栽培的常规过程之一。由于生物和非生物胁迫，苗圃条件下苗圃生产的扦插研究通常低于预期的生产力水平。提高苗圃产量仍然是葡萄研究人员的议程。封装的银纳米粒子 (AgNPs) 是通过绿色合成方法从 具有高酚类含量的葡萄籽提取物 ( Vitis vinifera L. cv Öküzgözü) 和硝酸银 (AgNO) 中制备的。AgNPs 的尺寸由透射电子显微镜 (TEM) 确定为 10-50 nm，并且没有团聚和褐色。AgNPs 对标准木质化 41B ( Vitis vinifera  L. cv. Chasselas ×  Vitis berlandieri)生根的影响) 含有吲哚 3 丁酸 (IBA) 的纯砧木（对照，0.5 mg L ^-1 AgNPs，0.5 mg L ^-1 AgNPs 50 ppm IBA，1 mg L ^-1 AgNPs，1 mg L ^-1 AgNPs 50 ppm IBA 和 50 ppm IBA) 进行了测试。通过显微断层扫描 (microCT) 评估了施用对温室 1:1 珍珠岩泥炭培养基中插条的影响、叶叶绿素含量、营养生长参数和对维管组织的影响。应用纯 AgNPs 并与 IBA 结合不会改变叶绿素含量。虽然 1 mg L ^-1 AgNPs 的应用是最接近 50 ppm IBA 的水平，但提供了根和芽的发育，而 1 mg L ^-1AgNPs 最能促进芽的发育。1 mg L ^-1 AgNPs + 50 ppm IBA 应用导致从插条发育的枝条中的节点数量最多。在对有根和无根葡萄藤插条的显微CT 分析中，确定了由于栓塞和育苗材料损失导致干燥导致的组织损伤。AgNPs 应用被认为可以提高葡萄砧木扦插的苗圃成功率。