Abstract

Surface modification is an emerging strategy for the design of contact materials. Fabricated alumina discs were functionalized by deposition of biogenic silver nanoparticles. The surfaces were characterized for physico-chemical, antibacterial and antibiofilm properties against microbial pathogens. The surface demonstrated improved hydrophobicity and a surface silver nanoparticle content of 6.4 w%. A reduction of more than 99.9% in CFU mL⁻ⁱ was observed against the Gram-positive and Gram-negative bacteria tested, with >90% reduction of the fungal isolate. After 4 h, microbial adhesion was reduced by >99.9 and 90% for Escherichia coli and Staphylococcus aureus, respectively. Scanning electron micrographs further revealed a biofilm reduction. Cell viability tests indicated a bioincompatibility higher than 80% with Caco-2 and HaCaT cell lines after 48 h contact. The results suggest that deposition of biogenic silver nanoparticles on the surface of contact materials could be employed as a strategy to prevent biofilm formation.

摘要

表面改性是一种新兴的接触材料设计策略。通过生物银纳米颗粒的沉积对制造的氧化铝盘进行功能化。对表面进行了物理化学、抗菌和抗微生物病原体抗生物膜特性的表征。该表面显示出改进的疏水性和 6.4 w% 的表面银纳米颗粒含量。观察到针对所测试的革兰氏阳性和革兰氏阴性细菌的CFU mL ^-i减少了 99.9%以上，其中真菌分离株减少了 90% 以上。4 小时后，大肠杆菌和金黄色葡萄球菌的微生物粘附减少 > 99.9 和 90% ，分别。扫描电子显微照片进一步显示生物膜减少。细胞活力测试表明，接触 48 小时后，与 Caco-2 和 HaCaT 细胞系的生物不相容性高于 80%。结果表明，生物银纳米粒子在接触材料表面的沉积可用作防止生物膜形成的策略。