Discoveries in model plants grown under optimal conditions can provide important directions for crop improvement. However, it is important to verify whether results can be translated to crop plants grown in the field. In this study, we sought to study the role of MYB28 in the regulation of aliphatic glucosinolate (A-GSL) biosynthesis and associated sulfur metabolism in field-grown Brassica oleracea with the use of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 gene-editing technology. We describe the first myb28 knockout mutant in B. oleracea, and the first CRISPR field trial in the United Kingdom approved and regulated by the UK Department for Environment, Food & Rural Affairs after the reclassification of gene-edited crops as genetically modified organisms by the European Court of Justice on July 25, 2018. We report that knocking out myb28 results in downregulation of A-GSL biosynthesis genes and reduction in accumulation of the methionine-derived glucosinolate, glucoraphanin, in leaves and florets of field-grown myb28 mutant broccoli plants, whereas accumulation of sulfate, S-methyl cysteine sulfoxide, and indole glucosinolate in leaf and floret tissues remained unchanged. These results demonstrate the potential of gene-editing approaches to translate discoveries in fundamental biological processes for improved crop performance.

中文翻译：

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MYB28 基因的 CRISPR-Cas9 介导的基因编辑会损害田间甘蓝的萝卜硫苷积累

在最佳条件下生长的模式植物的发现可以为作物改良提供重要方向。然而，重要的是要验证结果是否可以转化为在田间种植的作物。在这项研究中，我们试图使用成簇规则间隔短回文重复序列 (CRISPR)-Cas9 基因研究MYB28在调节田间种植的甘蓝中脂肪族芥子油苷 (A-GSL) 生物合成和相关硫代谢中的作用- 编辑技术。我们描述了甘蓝中的第一个myb28敲除突变体，以及在 2018 年 7 月 25 日欧洲法院将基因编辑作物重新归类为转基因生物后，英国环境、食品和农村事务部批准和监管的首次 CRISPR 现场试验。报道称，敲除myb28 会导致 A-GSL 生物合成基因的下调和甲硫氨酸衍生的硫代葡萄糖苷、萝卜硫苷在田间种植的myb28突变型西兰花植物的叶子和小花中的积累减少，而硫酸盐、S叶和小花组织中的甲基半胱氨酸亚砜和吲哚硫代葡萄糖苷保持不变。这些结果证明了基因编辑方法在转化基本生物过程中的发现以改善作物性能方面的潜力。