Cas9/guide RNA (gRNA)-based gene drive systems are expected to play a transformative role in malaria elimination efforts., whether through population modification, in which the drive system contains parasite-refractory genes, or population suppression, in which the drive system induces a severe fitness load resulting in population decline or extinction. DNA sequence polymorphisms representing alternate alleles at gRNA target sites may confer a drive-resistant phenotype in individuals carrying them. Modeling predicts that, for observed levels of SGV at potential target sites and observed rates of de novo DRA formation, population modification strategies are uniquely resilient to DRAs. We conclude that gene drives can succeed when fitness costs incurred by drive-carrying mosquitoes are low enough to prevent strong positive selection for DRAs produced de novo or as part of the SGV and that population modification strategies are less prone to failure due to drive resistance.

中文翻译：

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疟疾病媒控制的种群改造策略对观察到的基因驱动抗性等位基因水平具有独特的弹性

基于 Cas9/gRNA 的基因驱动系统有望在消除疟疾的努力中发挥变革性作用。导致严重的适应负荷，导致种群下降或灭绝。代表 gRNA 靶位点交替等位基因的 DNA 序列多态性可能会赋予携带它们的个体以驱动抗性表型。建模预测，对于观察到的潜在目标位点的 SGV 水平和观察到的从头 DRA 形成率，种群改造策略对 DRA 具有独特的弹性。