The immobilization of POXA1b laccase and its hydrophobin-fused chimera was performed at pristine Multiwalled Carbon Nanotube (MWCNT) and MWCNT-modified electrodes by electrografting of a 2-diazoniumanthraquinone salt. The influence of the hydrophobin domain and the MWCNT functionalization with anthraquinone groups towards immobilization of laccase was compared by direct electrochemistry under O₂ and electrochemical biosensing of phenols. The hydrophobin domain affords the stable dispersion of MWCNT in water/ethanol, while being detrimental to the direct electron transfer between POXA1b and the electrode. On the contrary, the stronger hydrophobic interactions between anthraquinone and laccase affords direct electrochemistry of POXA1b and enabled the design of a highly sensitive phenol biosensor, reaching a limit-of-detection (LOD) of 2 pM and sensitivity of 23,600 mA L mmol⁻¹ cm⁻² for catechol, and a LOD of 15 nM and sensitivity of 0.053 mA L mmol⁻¹ cm⁻² for dopamine.

通过电接枝 2-重氮蒽醌盐，在原始多壁碳纳米管 (MWCNT) 和 MWCNT 修饰的电极上固定 POXA1b 漆酶及其疏水蛋白融合嵌合体。在O ₂下通过直接电化学比较疏水蛋白结构域和具有蒽醌基团的MWCNT官能化对漆酶固定的影响和酚类的电化学生物传感。疏水蛋白域提供了 MWCNT 在水/乙醇中的稳定分散，同时不利于 POXA1b 和电极之间的直接电子转移。相反，蒽醌和漆酶之间更强的疏水相互作用提供了 POXA1b 的直接电化学，并能够设计出高灵敏度的苯酚生物传感器，检测限 (LOD) 为 2 pM，灵敏度为 23,600 mA L mmol ^-1 cm ^-2儿茶酚，LOD 为 15 nM，灵敏度为 0.053 mA L mmol ^-1 cm ^-2多巴胺。