Tolypothrix, a self-flocculating, fast growing, CO₂ and nitrogen-fixing cyanobacterium, can be cultivated in nutrient-poor ash dam waters of coal-fired power stations, converting CO₂ emissions into organic biomass. Therefore, the biomass of Tolypothrix sp. is a promising source for bio-fertiliser production, providing micro- and macronutrients. Energy requirements for production could potentially be offset via anaerobic digestion (AD) of the produced biomass, which may further improve the efficiency of the resulting biofertilizer. The aim of this study was to evaluate the effectiveness of pre-treatment conditions and subsequent methane (CH₄) production of Tolypothrix under out-door cultivation conditions. Pre-treatments on biogas and methane production for Tolypothrix sp. biomass investigated were: (1) thermal at 95 °C for 10 h, (2) hydrothermal by autoclave at 121 °C at 1013.25 hPa for 20 min, using a standard moisture-heat procedure, (3) microwave at an output power of 900 W and an exposure time of 3 min, (4) sonication at an output power of 10 W for 3.5 h at 10 min intervals with 20 s breaks and (5) freeze-thaw cycles at −80 °C for 24 h followed by thawing at room temperature. Thermal, hydrothermal and sonication pre-treatments supported high solubilization of organic compounds up to 24.40 g L⁻¹. However, higher specific CH₄ production of 0.012 and 0.01 L CH₄ g⁻¹ volatile solids_added. was achieved for thermal and sonic pre-treatments, respectively. High N- and low C-content of the Tolypothrix biomass affected CH₄ recovery, while pre-treatment accelerated production of volatile acids (15.90 g L⁻¹) and ammonia-N-accumulation (1.41 g L⁻¹), leading to poor CH₄ yields. Calculated theoretical CH₄ yields based on the elemental composition of the biomass were ~55% higher than actual yields. This highlights the complexity of interactions during AD which are not adequately represented by elemental composition.

Tolypothrix是一种自絮凝、快速生长、CO ₂和固氮蓝藻，可在燃煤电站的贫化灰坝水域中培养，将 CO ₂排放转化为有机生物质。因此，Tolypothrix sp的生物量。是生物肥料生产的有前途的来源，提供微量和常量营养素。生产的能源需求可能会通过生产的生物质的厌氧消化 (AD)来抵消，这可能会进一步提高所得生物肥料的效率。本研究的目的是评估的预处理条件和随后的甲烷（CH有效性₄）生产Tolypothrix在户外栽培条件下。Tolypothrix sp.沼气和甲烷生产的预处理。所研究的生物质是：（1）在 95 °C 下加热 10 小时，（2）在 1013.25 hPa 下通过​​高压釜在 121 °C 下进行水热 20 分钟，使用标准湿热程序，（3）微波输出功率为900 W 和 3 分钟的暴露时间，(4) 以 10 W 的输出功率超声处理 3.5 小时，间隔 10 分钟，间隔 20 秒，(5) 在 -80 °C 下冻融循环 24 小时，然后是在室温下解冻。热、水热和超声预处理支持高达 24.40 g L ^-1的有机化合物的高溶解度。然而，更高的特定 CH ₄产量为 0.012 和 0.01 L CH ₄ g ^-1_添加挥发性固体_。分别用于热处理和声波预处理。Tolypothrix生物质的高N - 和低C -含量影响 CH ₄回收，而预处理加速了挥发性酸（15.90 g L ^-1）的产生和氨-N -积累（1.41 g L ^-1），导致不良反应CH ₄产率。基于生物质的元素组成计算的理论 CH ₄产量比实际产量高约 55%。这突出了 AD 期间相互作用的复杂性，这些相互作用并没有被元素组成充分代表。