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The association of transcription factor Prox1 with the proliferation, migration, and invasion of lung cancer
Open Life Sciences ( IF 2.2 ) Pub Date : 2021-01-01 , DOI: 10.1515/biol-2021-0056
Xinxin Hao 1, 2 , Wenting Luo 3 , Xueshan Qiu 1
Affiliation  

Background The current study investigates the effect of transcription factor Prox1 on the proliferation, migration, and invasion ability of lung cancer. Methods Lung cancer cell lines (A549 and H446 cells) were transfected with Prox1NAD and siRNA, respectively. Thus, the A549 and H446 cells overexpressed Prox1 after transfection of Prox1NAD plasmids, and A549 and H446 cells have low expression of Prox1 after transfection with siRNA. Reverse transcriptase quantitative PCR and western blot analyses were used to detect Prox1 mRNA and protein expression in cells. Plate clone formation experiments and MTT experiments were used to detect cell proliferation. Western blot was used to detect the expression of Rho family-related proteins in cells. Results Compared to untransfected wild-type A549 and H446 that served as blank controls, the expression level of Prox1mRNA and protein in A549 and H446 cells overexpressing Prox1 after plasmid transfection was high, while the expression level of Prox1mRNA and protein in A549 and H446 cells with low expression of Prox1 after siRNA transfection was low. With the increase of Prox1 expression, the expression of RhoA and RhoC increased, while the expression of RhoB decreased. Conclusion The finding of this study may provide a new approach for the treatment of lung cancer using targeted gene therapy.

中文翻译:

转录因子Prox1与肺癌增殖、迁移和侵袭的关系

背景 本研究探讨转录因子Prox1对肺癌增殖、迁移和侵袭能力的影响。方法分别用Prox1NAD和siRNA转染肺癌细胞系(A549和H446细胞)。因此,A549和H446细胞转染P​​rox1NAD质粒后Prox1过表达,A549和H446细胞转染siRNA后Prox1低表达。逆转录酶定量 PCR 和蛋白质印迹分析用于检测细胞中 Prox1 mRNA 和蛋白质的表达。平板克隆形成实验和MTT实验用于检测细胞增殖。Western blot检测细胞内Rho家族相关蛋白的表达。结果与作为空白对照的未转染的野生型 A549 和 H446 相比,质粒转染后Prox1过表达的A549和H446细胞中Prox1mRNA和蛋白的表达水平较高,而siRNA转染后Prox1低表达的A549和H446细胞中Prox1mRNA和蛋白的表达水平较低。随着Prox1表达的增加,RhoA和RhoC的表达增加,而RhoB的表达减少。结论 本研究的发现可能为靶向基因治疗肺癌的治疗提供一种新的途径。而 RhoB 的表达下降。结论 本研究的发现可能为靶向基因治疗肺癌的治疗提供一种新的途径。而 RhoB 的表达下降。结论 本研究的发现可能为靶向基因治疗肺癌的治疗提供一种新的途径。
更新日期:2021-01-01
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