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Satellite DNA probes of Alstroemeria longistaminea (Alstroemeriaceae) paint the heterochromatin and the B chromosome, reveal a G-like banding pattern, and point to a strong structural karyotype conservation
Protoplasma ( IF 2.9 ) Pub Date : 2021-06-20 , DOI: 10.1007/s00709-021-01681-7
Tiago Ribeiro 1, 2 , Magdalena Vaio 3 , Leonardo P Félix 4 , Marcelo Guerra 1
Affiliation  

Alstroemeria species present a well-conserved and asymmetric karyotype. The genus is divided into a Chilean clade, rich in heterochromatin, and a Brazilian clade, poor in heterochromatin. We investigated the distribution of the main repetitive sequences in the chromosomes of the Brazilian species A. longistaminea (2n = 16 + 0-6B) aiming to evaluate the role played by these sequences on the structural organization of the karyotype. In situ hybridization of the three most abundant retrotransposons, corresponding to ~ 45% of the genome, was uniformly distributed. Three satellite DNA sequences, representing near half of the whole satellite fraction (1.93% of the genome), were mainly concentrated on the heterochromatin and one of them painted the whole B chromosome. Noteworthy, some satellites were located on euchromatin, either dispersed or concentrated in clusters along the chromosomes, revealing a G-band-like pattern. The two satellites that presented more C-band- and G-band-like labeling were also hybridized in situ in two other Alstroemeria species. They revealed astonishing similar patterns of distribution, indicating an unusually structural karyotype conservation among Brazilian species.



中文翻译:

Alstroemeria longistaminea (Alstroemeriaceae) 的卫星 DNA 探针描绘了异染色质和 B 染色体,揭示了 G 样条带模式,并指出了强烈的结构核型保守性

六出属物种呈现出保守且不对称的核型。该属分为富含异染色质的智利进化枝和缺乏异染色质的巴西进化枝。我们调查了巴西物种A. longistaminea (2 n= 16 + 0-6B) 旨在评估这些序列对核型结构组织所起的作用。三个最丰富的反转录转座子的原位杂交,对应于约 45% 的基因组,是均匀分布的。三个卫星 DNA 序列,占整个卫星部分的近一半(基因组的 1.93%),主要集中在异染色质上,其中一个描绘了整个 B 染色体。值得注意的是,一些卫星位于常染色质上,沿着染色体分散或集中成簇,显示出类似 G 带的模式。呈现更多 C 波段和 G 波段标记的两颗卫星也在另外两颗六出花中原位杂交物种。他们揭示了惊人的相似分布模式,表明巴西物种之间存在异常的结构核型保护。

更新日期:2021-06-20
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