The coffee berry borer (CBB), Hypothenemus hampei, is the major insect pest of coffee crops worldwide. Coffee berry borer females use coffee berry volatiles during host-searching behaviour. Nonetheless, conspecific infestation is a factor that may affect coffee berry volatile profile and so the attraction of CBB females to coffee berries. In this study, we evaluated the attraction of CBB females to volatiles from healthy Coffea canephora berries and three different conspecific infestation levels in laboratory and field assays. We used solid-phase microextraction coupled with gas chromatography–mass spectrometry to identify the volatile compounds in the effluvia of coffee berries. Overall, our laboratory bioassays showed that CBB females were more attracted to the volatiles emitted by healthy berries and those with early and medium infestation levels than to late-infested berries. The field assay showed that traps baited with healthy berries or berries with early infestations attracted more CBB females than those baited with berries with the late infestations. The chemical analyses showed that healthy berries emitted 21 compounds, with ethyl isovalerate, ∝-pinene camphene, β-pinene, and limonene in higher amounts. Berries with an early infestation emitted 22 compounds including methyl salicylate, trans-β-ocimene, and linalool as the major compounds. Berries with a medium infestation released 15 compounds with methyl salicylate, trans-β-ocimene, and an unknown monoterpenoid in higher amounts. Berries with late infestation emitted only six compounds with tetradecane, tridecane, and dodecanol as the major compounds. The results of this study showed that the conspecific infestation of robusta coffee berries affects CBB female attraction.

咖啡浆果螟 (CBB) Hypothenemus hampei是世界范围内咖啡作物的主要害虫。咖啡浆果蛀虫雌性在寻找宿主的过程中会使用咖啡浆果挥发物。尽管如此，同种侵染是一个可能影响咖啡浆果挥发性特征的因素，因此 CBB 雌性对咖啡浆果的吸引力。在这项研究中，我们评估了 CBB 雌性对健康咖啡豆挥发物的吸引力浆果和实验室和田间试验中三种不同的同种侵染水平。我们使用固相微萃取结合气相色谱-质谱法来鉴定咖啡浆果臭气中的挥发性化合物。总体而言，我们的实验室生物测定表明，与晚期感染的浆果相比，CBB 雌性更容易被健康浆果和具有早期和中期侵染水平的浆果释放的挥发物所吸引。田间试验表明，以健康浆果或早期侵染浆果为诱饵的陷阱比以晚期侵染浆果为诱饵的陷阱吸引了更多的 CBB 雌性。化学分析表明，健康的浆果会释放 21 种化合物，其中异戊酸乙酯、∝-蒎烯、β-蒎烯和柠檬烯的含量更高。早期侵染的浆果释放出 22 种化合物，包括水杨酸甲酯、反式-β-罗勒烯和芳樟醇作为主要化合物。中等侵染的浆果释放出 15 种化合物，其中包括水杨酸甲酯、反式 β-罗勒烯和一种未知的单萜类化合物。晚侵染的浆果仅释放六种化合物，其中主要化合物为十四烷、十三烷和十二烷醇。这项研究的结果表明，罗布斯塔咖啡浆果的同种感染会影响 CBB 女性的吸引力。和十二醇为主要化合物。这项研究的结果表明，罗布斯塔咖啡浆果的同种感染会影响 CBB 女性的吸引力。和十二醇为主要化合物。这项研究的结果表明，罗布斯塔咖啡浆果的同种感染会影响 CBB 女性的吸引力。