Asparagus decline syndrome (ADS), one of the most important diseases affecting asparagus crops, causes important yield losses worldwide. Fusarium proliferatum, F. oxysporum and F. redolens are among the main species associated with ADS. To explore their potential inoculum sources and the effectiveness of soil disinfestation practices for ADS management, molecular methods based on a quantitative real-time polymerase chain reaction (qPCR) were developed. qPCR-based molecular tools demonstrated advantages in the sensitive and specific detection and quantification of fungal pathogens in comparison with less-accurate and time-consuming traditional culture methods.

中文翻译：

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芦笋衰退综合征中镰刀菌属物种的潜在接种源以及通过 qPCR 协​​议评估土壤灭虫方法

芦笋衰退综合症 (ADS) 是影响芦笋作物的最重要疾病之一，在全球范围内造成了重大的产量损失。Fusarium proliferatum、F. oxysporum和F. redolens是与 ADS 相关的主要物种。为了探索它们的潜在接种源和土壤消毒实践对 ADS 管理的有效性，开发了基于定量实时聚合酶链反应 (qPCR) 的分子方法。与精度较低且耗时的传统培养方法相比，基于 qPCR 的分子工具在真菌病原体的灵敏和特异性检测和定量方面具有优势。