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Multilocus DNA polymorphism of some rubber-bearing dandelions (Taraxacum spp.) of Russia and Kazakhstan
Genetic Resources and Crop Evolution ( IF 2 ) Pub Date : 2021-06-19 , DOI: 10.1007/s10722-021-01233-1
Bari Gabit , Gainullina Karina , Gumerova Gulnar , Uteulin Kairat , Golovanov Yaroslav , Chemeris Aleksey , Kuluev Bulat

Successful domestication and introduction into culture of new forms of rubber-bearing dandelions require not only determination of the content and quality of rubber in them, but also accurate species identification and genetic certification of valuable lines and forms. The objects of our study were three samples of Taraxacum kok-saghyz Rodin KAZ08-023, L2, Sary Jaz, brown-seeded and pink-seeded forms of Taraxacum hybernum Stev., as well as along one sample of Taraxacum brevicorniculatum Korol., Taraxacum glaucanthum (Ledeb.) DC., and Taraxacum officinale L. The aim of our work was to search of RAPD, ISSR, and SSR markers for the unambiguous identification of all studied dandelion forms. The RAPD primers OPD-07 and FS-10 are best suited for the detection of interspecific polymorphism, while the RAPD primers FS-10, OPC-05 and OPC-08 can be used for the detection of intraspecific dandelion polymorphism. The ISSR primers HB10, ISSR17, and ISSR36 were most effective for detecting interspecific polymorphism. For identification of the brown-seeded and pink-seeded forms of T. hybernum, primers HB10 and ISSR38 can be used. Primers HB10 and ISSR14 are best suited for identifying the three studied kok-saghyz samples. SSR primers TKS0091, TKS0097, TKS0107, and TKS0110 are suitable for SSR identification of pink-seeded and brown-seeded forms of T. hybernum. Only locus TKS0111 is suitable for unambiguous SSR identification of all three studied kok-saghyz samples.



中文翻译:

俄罗斯和哈萨克斯坦一些含橡胶蒲公英(Taraxacum spp.)的多位点DNA多态性

新品种含胶蒲公英的成功驯化和引入养殖不仅需要确定其中橡胶的含量和质量,还需要准确的物种鉴定和有价值的品系和形式的遗传认证。我们研究的对象是三个样品橡胶草罗丹KAZ08-023,L2,萨利贾兹,棕色种子和粉红色种子形式的蒲公英hybernum甜菊,以及沿的一个样本蒲公英brevicorniculatum Korol,蒲公英glaucanthum (Ledeb.) DC. 和蒲公英L. 我们工作的目的是搜索 RAPD、ISSR 和 SSR 标记,以明确识别所有研究的蒲公英形式。RAPD 引物 OPD-07 和 FS-10 最适合检测种间多态性,而 RAPD 引物 FS-10、OPC-05 和 OPC-08 可用于检测种内蒲公英多态性。ISSR 引物 HB10、ISSR17 和 ISSR36 对检测种间多态性最有效。可以使用引物 HB10 和 ISSR38 来鉴定棕色种子和粉红色种子形式的T. hybernum。引物 HB10 和 ISSR14 最适合识别三个研究的 kok-sa​​ghyz 样本。SSR 引物 TKS0091、TKS0097、TKS0107 和 TKS0110 适用于 SSR 鉴定粉红色种子和棕色种子形式的T. hybernum。 只有基因座 TKS0111 适用于所有三个研究的 kok-sa​​ghyz 样本的明确 SSR 识别。

更新日期:2021-06-19
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