We present a densitometric quantification method for triclosan in toothpaste, separated by high-performance thin-layer chromatography (HPTLC) and using a 48-bit flatbed scanner as the detection system. The sample was band-wise applied to HPTLC plates (10 × 20 cm), with fluorescent dye, Merck, Germany (1.05554). The plates were developed in a vertical developing chamber with 20 min of chamber saturation over 70 mm, using n-heptane–methyl tert-butyl ether–acetic acid (92:8:0.1, V/V) as solvent. The R_F value of triclosan is hR_F = 22.4, and quantification is based on direct measurements using an inexpensive 48-bit flatbed scanner for color measurements (in red, green, and blue) after plate staining with 2,6-dichloroquinone-4-chloroimide (Gibbs' reagent). Evaluation of the red channel makes the measurements of triclosan very specific. For linearization, an extended Kubelka–Munk expression was used for data transformation. The range of linearity covers more than two orders of magnitude and is between 91 and 1000 ng. The separation method is inexpensive, fast and reliable.

我们提出了一种牙膏中三氯生的密度定量方法，通过高效薄层色谱 (HPTLC) 分离，并使用 48 位平板扫描仪作为检测系统。将样品带状应用于 HPTLC 板 (10 × 20 cm)，荧光染料，默克，德国 (1.05554)。平板在垂直显影室中显影，室饱和度超过 70 毫米，20 分钟，使用正庚烷-甲基叔丁基醚-乙酸（92:8:0.1，V/V）作为溶剂。三氯生的R _F值为hR _F = 22.4，量化是基于使用廉价的 48 位平板扫描仪进行颜色测量（红色、绿色和蓝色）后用 2,6-二氯醌-4-氯酰亚胺（吉布斯试剂）染色后的直接测量。红色通道的评估使得三氯生的测量非常具体。对于线性化，扩展的Kubelka-Munk表达式用于数据转换。线性范围涵盖两个以上的数量级，介于 91 和 1000 ng 之间。该分离方法廉价、快速且可靠。