Amisulbrom (AML), a sulfonamide fungicide used to control oomycete diseases, is regarded as a threat to aquatic species. The objective of this study was to evaluate the potential effects of AML on fish using a zebrafish model. Zebrafish embryos were exposed to 0.0075 μM, 0.075 μM, and 0.75 μM AML. AML-treated zebrafish embryos exhibited severe developmental defects, including pericardial edema, blood-clot clustering, increased hatching rates, decreased heart rates, and abnormal hemoglobin distributions. Compared with controls, key marker genes associated with cardiovascular development (i.e., nkx2.5, myh6, myh7, myl7, alas2, hbbe1, hbbe2, and gata1a) were abnormally expressed in response to AML treatment, suggesting that AML might specifically affect cardiovascular development. These results provide a valuable reference for the effects of AML on zebrafish embryos and may help to further clarify the potential risks posed by AML to aquatic ecosystems.

氨磺草胺 (AML) 是一种用于控制卵菌病的磺胺类杀菌剂，被视为对水生物种的威胁。本研究的目的是使用斑马鱼模型评估 AML 对鱼类的潜在影响。斑马鱼胚胎暴露于 0.0075 μM、0.075 μM 和 0.75 μM AML。AML 处理的斑马鱼胚胎表现出严重的发育缺陷，包括心包水肿、血凝块聚集、孵化率增加、心率降低和血红蛋白分布异常。与对照组相比，与心血管发育相关的关键标记基因（即nkx2.5、myh6、myh7、myl7、alas2、hbbe1、hbbe2和gata1 a ) 在 AML 治疗中异常表达，表明 AML 可能特别影响心血管发育。这些结果为AML对斑马鱼胚胎的影响提供了有价值的参考，并可能有助于进一步阐明AML对水生生态系统构成的潜在风险。