Abstract

Common purslane that is a natural source of antioxidants, phenols, flavonoids, and dopamine possesses the potential to be used as a medicinal vegetable. The amount of active compounds in purslane is naturally low, so elicitation of plant response to produce more secondary metabolites may be important. In order to increase the amount of medicinal substances of the common purslane, foliar application and fertilization of silicon (each of them in two concentrations: 1 and 2 mM) alongside with methyl jasmonate spray (0, 75 and 150 µM) were applied. Measurement of silicon showed that the highest amount of leaf silicon was seen in 1 mM silicon spray regardless of methyl jasmonate treatments. Spraying of silicon without methyl jasmonate (Ss₁M₀) increased the leaf weight, grain yield, total phenol and leaf flavonoid content. Measurement of dopamine using high-performance liquid chromatography showed that fertilization of 1 mM silicon (Sf₁M₀) in the treatment group increased dopamine up to 2.5 folds in comparison with the control group. Also, silicon fertilization increased photosynthetic pigments and phosphorus concentration of leaves. To conclude, silicon can be used as an elicitor for increasing the amount of dopamine because its effect on dopamine biosynthesis was significantly more than methyl jasmonate that is a general elicitor. The effects of silicon on other medicinal substances of common purslane can be another avenue for further research.

摘要

马齿苋是抗氧化剂、酚类、类黄酮和多巴胺的天然来源，具有用作药用蔬菜的潜力。马齿苋中活性化合物的含量自然很低，因此引发植物反应以产生更多次生代谢物可能很重要。为了增加马齿苋药用物质的量，叶面施肥和硅施肥（每种浓度为 1 和 2  mM）以及茉莉酸甲酯喷雾剂（0、75 和 150 µM）。硅的测量表明，无论茉莉酸甲酯处理如何，在 1 mM 硅喷雾中都能看到最高含量的叶子硅。喷涂不含茉莉酸甲酯的硅 (Ss ₁ M ₀) 增加了叶重、谷物产量、总酚和叶黄酮含量。使用高效液相色谱法测量多巴胺表明，与对照组相比，处理组中 1 mM 硅 (Sf ₁ M ₀ ) 的施肥使多巴胺增加了 2.5 倍。此外，硅肥增加了叶片的光合色素和磷浓度。总而言之，硅可以用作增加多巴胺量的诱导剂，因为它对多巴胺生物合成的影响明显大于作为一般诱导剂的茉莉酸甲酯。硅对马齿苋其他药用物质的影响可以成为进一步研究的另一条途径。