Glycoside hydrolase (GH, EC 3.2.1) is a group of enzymes that hydrolyzes glycosidic bonds and play a role in the hydrolysis and synthesis of sugars in living organisms. Vitis vinifera is an important fruit crop and it harbors GH17 gene family however, their function in grapes has not been systematically investigated. In this study, a total of 870 GH17 genes were identified from 14 plant species and their structural domain, sequence alignment, phylogenetic tree, collinear analysis, with the expression profiles of VviGH17 gene family was performed. The promoter analysis of VviGH17 gene showed the presence of cis-acting elements, which are responsive to plant growth and development. In addition, elements for plant hormones were found that are triggered in response to abiotic/biological stress. Transcriptomic data led to the identification of several VviGH17 genes, which are associated with bud dormancy and in response to abiotic stress. Transcript analysis was carried out for some of the selected VviGH17 genes RT-qPCR. VviGH17-16 and VviGH17-30 genes were differentially expressed during bud dormancy, fruit development and different abiotic stresses. Moreover, VviGH17-37 and VviGH17-44 were differentially expressed at fruit development, in response to abiotic stress. In addition, subcellular localization predicts that the VviGH17-16, VviGH17-30, and VviGH17-37 genes were located in the cell membrane, while VviGH17-44 gene was located in the vacuole. In conclusion, our study led to the identification of several GH17s and their probable role in development and stress.

糖苷水解酶（GH，EC 3.2.1）是一组水解糖苷键的酶，在生物体内糖的水解和合成中发挥作用。葡萄是一种重要的果树作物，含有GH17基因家族，但其在葡萄中的功能尚未得到系统研究。本研究从14个植物物种中总共鉴定了870个GH17基因，并对其结构域、序列比对、系统发育树、共线分析以及VviGH17基因家族的表达谱进行了分析。VviGH17基因的启动子分析表明存在顺式作用元件，这些元件对植物生长和发育有反应。此外，还发现植物激素元素可以响应非生物/生物胁迫而触发。转录组数据导致了几个VviGH17基因的鉴定，这些基因与芽休眠和对非生物胁迫的反应有关。对一些选定的VviGH17基因进行RT-qPCR 转录本分析。VviGH17-16和VviGH17-30基因在芽休眠、果实发育和不同非生物胁迫期间差异表达。此外，VviGH17-37和VviGH17-44在果实发育过程中差异表达，以响应非生物胁迫。此外，亚细胞定位预测VviGH17-16、VviGH17-30和VviGH17-37基因位于细胞膜中，而VviGH17-44基因位于液泡中。总之，我们的研究鉴定了几种GH17及其在发育和应激中可能的作用。