Bilirubin, a breakdown product of hemoglobin, is a remarkable marker for diagnosis of hemolytic disorders. In a healthy human 200 to 300 mg of bilirubin is produced and excreted per day. Bilirubin concentration ranges from 0.3 to 1.9 mg/100 mL of blood. Low bilirubin concentration in the blood is coupled with high risk of coronary heart diseases and iron deficiency. Serum bilirubin level above than 2.5 mg/100 mL, leads to hyperbilirubinemia. Therefore, measurement of serum bilirubin level is of great significance. Present review highlights various methods for analysis of bilirubin, with special insistence on bio-sensing methods. Till now, a variety of nanomaterials have been exploited for improvement of different biosensors during the past few decades. The future research, therefore, could be spotlighted on the miniaturization of bilirubin biosensors by reconciling the properties of interface with nanoparticles through engineering morphology, particle size, effective surface area, functionality, adsorption capability and electron-communication properties.

胆红素是血红蛋白的一种分解产物，是诊断溶血性疾病的重要标志物。健康人每天会产生和排出 200 至 300 毫克胆红素。胆红素浓度范围为 0.3 至 1.9 毫克/100 毫升血液。血液中的低胆红素浓度与冠心病和缺铁的高风险相结合。血清胆红素水平高于 2.5 mg/100 mL，会导致高胆红素血症。因此，测定血清胆红素水平具有重要意义。本综述重点介绍了分析胆红素的各种方法，特别强调了生物传感方法。到目前为止，在过去的几十年中，已经开发了多种纳米材料来改进不同的生物传感器。因此，未来的研究，