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Optimization of preparation and properties of Gardenia yellow pigment-loaded alginate beads
Korean Journal of Chemical Engineering ( IF 2.7 ) Pub Date : 2021-06-18 , DOI: 10.1007/s11814-021-0807-3
Yong Liu , Qing Zhou , Yan-Mei He , Xiu-Yun Ma , Lin-Na Liu , Yong-Jian Ke

Gardenia yellow pigment (GYP) loaded alginate beads were prepared by the ionic gelation technique, and the preparation parameters were optimized by response surface methodology for high encapsulation efficiency. The optimized parameters were alginate concentration of 3.3%, CaCl2 concentration of 2.4%, and GYP concentration of 3.2 mg/mL, under which the encapsulation efficiency was 73.61%. The surface morphology and bead size analysis showed that the GYP-loaded alginate beads had a roughly spherical morphology with a wrinkled surface, and their average diameter was 0.87 mm. In vitro release test revealed that the GYP release had a pH-dependent release profile and a two-step release process. The Rigter-Peppas model was the most proper model to assess the GYP release from alginate beads. The release mechanism of GYP at pH 1.2 and 7.4 was non-Fickian transport and case-II transport, respectively. The 2,2-diphenyl-1-picrylhydrazyl assay indicated that the encapsulated GYP had effectively maintained 82.56% of the antioxidant activity.



中文翻译:

栀子黄色素海藻酸盐微珠的制备及性能优化

采用离子凝胶技术制备了栀子黄色素(GYP)负载海藻酸盐微球,并通过响应面法优化了制备参数以获得高包封率。优化参数为海藻酸盐浓度3.3%,CaCl 2浓度为2.4%,GYP浓度为3.2 mg/mL,包封率为73.61%。表面形貌和珠粒尺寸分析表明,负载 GYP 的海藻酸盐珠粒具有大致球形的形态,表面有皱纹,平均直径为 0.87 mm。体外释放试验表明 GYP 释放具有 pH 依赖性释放曲线和两步释放过程。Rigter-Peppas 模型是评估海藻酸盐珠粒释放 GYP 的最合适模型。GYP 在 pH 1.2 和 7.4 时的释放机制分别是非 Fickian 转运和 case-II 转运。2,2-diphenyl-1-picrylhydrazyl 测定表明,包封的 GYP 有效地保持了 82.56% 的抗氧化活性。

更新日期:2021-06-18
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