Insect specimens morphologically similar to bed bugs were collected from Indigenous Tribe (Orang Asli) Village RPS Kemar, Perak, Malaysia. The insects were tentatively identified as Cimex hemipterus based on the measurement of the ratio of width and height of pronotum. To further confirm the species of the insects, parts of the Cytochrome Oxidase subunit I (CO1) and the 16S rRNA (16S) genes were amplified and sequenced. The partial sequences of CO1 and 16S genes showed high similarity with the percentage 99.54–100.00% to C. hemipterus sequences on BLAST. The Maximum Likelihood (ML) tree constructed using MEGA7 also showed that the sequences obtained were clustered together with C. hemipterus reference sequences retrieved from GenBank, with bootstrap values of 99% for the CO1 tree and 100% for the 16S tree, thus confirmed the species identification as C. hemipterus. Genetic diversity analysis using CO1 sequences showed that the bed bugs population in RPS Kemar has two haplotypes and low nucleotide diversities, while only one haplotype was found for 16S sequences, indicate no nucleotide diversity. The CO1 sequences in this study were also compared with CO1 sequences of C. hemipterus from other states in Malaysia. The results showed that the two haplotypes detected from RPS Kemar have no significant difference with the other haplotype groups from other states in Malaysia, suggesting that the Orang Asli Village RPS Kemar population could be a tropical bed bug population expansion.

从马来西亚霹雳州的土著部落 (Orang Asli) RPS Kemar 村收集在形态上与臭虫相似的昆虫标本。根据前胸膜的宽高比，初步鉴定为半翅目昆虫。为了进一步确认昆虫的种类，我们扩增并测序了部分细胞色素氧化酶亚基 I (CO1) 和 16S rRNA (16S) 基因。CO1和16S基因的部分序列与BLAST上的C. hemipterus序列显示出99.54-100.00%的高度相似性。使用 MEGA7 构建的最大似然 (ML) 树也表明获得的序列与C. hemipterus 聚类在一起从 GenBank 检索到的参考序列，CO1 树的 bootstrap 值为 99%，16S 树的 bootstrap 值为 100%，从而确认物种鉴定为C. hemipterus。使用CO1序列的遗传多样性分析表明，RPS Kemar的臭虫种群有两种单倍型，核苷酸多样性较低，而16S序列仅发现一种单倍型，表明没有核苷酸多样性。本研究中的 CO1 序列也与C. hemipterus 的CO1 序列进行了比较来自马来西亚其他州。结果表明，从RPS Kemar 检测到的两种单倍型与来自马来西亚其他州的其他单倍型群体没有显着差异，表明Orang Asli Village RPS Kemar 种群可能是热带臭虫种群的扩张。