While promising, the efficacy of aggregation-induced emission (AIE)-based photodynamic therapy (PDT) is limited by several factors including limited depth of laser penetration and intratumoral hypoxia. In the present study, a novel bacteria-based AIEgen (TBP-2) hybrid system (AE) is developed, that is able to facilitate the hypoxia-tolerant PDT treatment of orthotopic colon tumors via an interventional method. For this approach, an interventional device is initially designed, composed of an optical fiber and an endoscope, allowing for clear visualization of the position of the orthotopic tumor within the abdominal cavity. It is then possible to conduct successful PDT treatment of this hypoxic tumor via laser irradiation, as the TBP-2 is able to generate hydroxyl radicals (•OH) via a type I mechanism within this hypoxic microenvironment. Moreover, this interventional approach is proved to significantly impair orthotopic colon cancer growth and overcame PDT defects. This study is the first report involving such an interventional PDT strategy to knowledge, and it has the potential to complement other treatment modalities while also highlighting novel approaches to the design of hybrid AIEgen systems.

中文翻译：

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明亮细菌通过介入方法对原位结肠肿瘤进行耐缺氧光动力治疗

虽然前景广阔，但基于聚集诱导发射（AIE）的光动力疗法（PDT）的疗效受到多种因素的限制，包括激光穿透深度有限和瘤内缺氧。在本研究中，开发了一种新型基于细菌的AIEgen（TBP-2）混合系统（AE），能够通过介入方法促进原位结肠肿瘤的耐缺氧PDT治疗。对于这种方法，最初设计了一种介入装置，由光纤和内窥镜组成，可以清晰地显示腹腔内原位肿瘤的位置。然后可以通过激光照射对这种缺氧肿瘤进行成功的PDT治疗，因为TBP-2能够在这种缺氧微环境中通过I型机制产生羟基自由基(·OH)。此外，这种介入方法被证明可以显着抑制原位结肠癌的生长并克服 PDT 缺陷。这项研究是第一份涉及这种介入性 PDT 策略的报告，它有可能补充其他治疗方式，同时也强调混合 AIEgen 系统设计的新方法。