Background

Phosphoinositide-specific phospholipase C proteins mediate environmental stress responses in many plants. However, the potential of PI-PLC genes involved with abiotic stress tolerance in wheat remains un-explored.

Objective

To study TaPLC1 genetic relation with wheat drought and heat resistance.

Methods

The seedlings were treated with PI-PLC inhibitor U73122 at the single leaf stage. The seedlings were treated with drought and heat stress at the two leaf stage, and some physiological indexes and the expression profile of TaPLC1 gene were determined. And the TaPLC1 overexpression vector was transferred to Arabidopsis and selected to T3 generation for drought and heat stress treatment.

Results

After 4 h of drought and heat stress, the SOD activity, MDA and soluble sugar content of the two cultivars with inhibitor were higher than those without inhibitor, the chlorophyll content decreased. CS seedlings showed significant wilting phenomenon, and TAM107 showed slight wilting. After the elimination of drought and heat stress, all seedling wilting gradually recovered, while the leaf tips of the two varieties treated with inhibitors began to wilt and turn yellow, which was more significant 5 days after the drought and heat stress, while the degree of spring wilting and yellow in CS was earlier than that in TAM107. The expression patterns of TaPLC1 gene were different in the two cultivars, but the expression levels reached the maximum at 30 min of heat stress. The change of TaPLC1 expression in TAM107 without inhibitor treatment was significantly greater than that in CS. The expression level of TaPLC1 in the two cultivars under stress was significantly different between the two cultivars treated with inhibitor and untreated, and was lower than that of the normal plants under normal conditions. These results indicated that inhibition of TaPLC1 gene expression could enhance the sensitivity of seedlings to stress. In Arabidopsis, the root lengths of transgenic and wild-type seedlings were shortened after drought stress treatment, but the root lengths of transgenic plants decreased slightly. And the expression of TaPLC1 gene was significantly increased after drought and heat stress. This indicated that overexpression of TaPLC1 improved drought resistance of Arabidopsis.

Conclusions

The results of this study suggest that TaPLC1 may be involved in the regulation mechanism of drought and heat stress in wheat.

中文翻译：

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参与小麦耐热耐旱性的磷脂酶 C 基因 (Triticum aestivum L.)

背景

磷酸肌醇特异性磷脂酶 C 蛋白在许多植物中介导环境应激反应。然而，涉及小麦非生物胁迫耐受性的PI-PLC基因的潜力仍未得到探索。

客观的

研究TaPLC1与小麦干旱耐热性的亲缘关系。

方法

幼苗在单叶期用PI-PLC抑制剂 U73122 处理。在两叶期对幼苗进行干旱和热胁迫处理，并测定了一些生理指标和TaPLC1基因的表达谱。并将TaPLC1过表达载体转移至拟南芥并选择T3代进行干旱和热胁迫处理。

结果

干旱和热胁迫4 h后，加抑制剂的两个品种的SOD活性、MDA和可溶性糖含量均高于未加抑制剂的品种，叶绿素含量降低。CS幼苗出现明显的萎蔫现象，TAM107出现轻微的萎蔫现象。干旱和热胁迫消除后，所有幼苗萎蔫逐渐恢复，而抑制剂处理的两个品种叶尖开始萎蔫变黄，干旱和热胁迫后5 d更为显着。 CS春季萎黄早于TAM107。TaPLC1基因在两个品种中的表达模式不同，但在热应激30 min时表达水平达到最大值。TaPLC1的变化在没有抑制剂处理的 TAM107 中的表达明显高于在 CS 中的表达。TaPLC1在胁迫下的两个品种中的表达水平在抑制剂处理和未处理的两个品种之间存在显着差异，并且低于正常条件下的正常植物。这些结果表明，抑制TaPLC1基因表达可以增强幼苗对胁迫的敏感性。在拟南芥中，转基因和野生型幼苗在干旱胁迫处理后根长缩短，但转基因植株根长略有下降。以及TaPLC1的表达干旱和热应激后基因显着增加。这表明TaPLC1的过表达提高了拟南芥的抗旱性。

结论

本研究结果提示TaPLC1可能参与小麦干旱和热胁迫的调控机制。