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Combination of 15N Tracer and Microbial Analyses Discloses N2O Sink Potential of the Anammox Community
Environmental Science & Technology ( IF 11.4 ) Pub Date : 2021-06-18 , DOI: 10.1021/acs.est.1c00674
Toshikazu Suenaga 1 , Takumi Ota 2 , Kohei Oba 2 , Kentaro Usui 2 , Toshiki Sako 2 , Tomoyuki Hori 3 , Shohei Riya 1, 2 , Masaaki Hosomi 2 , Kartik Chandran 4 , Susanne Lackner 5 , Barth F Smets 1, 6 , Akihiko Terada 1, 2
Affiliation  

Although nitrogen removal by partial nitritation and anammox is more cost-effective than conventional nitrification and denitrification, one downside is the production and accumulation of nitrous oxide (N2O). The potential exploitation of N2O-reducing bacteria, which are resident members of anammox microbial communities, for N2O mitigation would require more knowledge of their ecophysiology. This study investigated the phylogeny of resident N2O-reducing bacteria in an anammox microbial community and quantified individually the processes of N2O production and N2O consumption. An up-flow column-bed anammox reactor, fed with NH4+ and NO2 and devoid of oxygen, emitted N2O at an average conversion ratio (produced N2O: influent nitrogen) of 0.284%. Transcriptionally active and highly abundant nosZ genes in the reactor biomass belonged to the Burkholderiaceae (clade I type) and Chloroflexus genera (clade II type). Meanwhile, less abundant but actively transcribing nosZ strains were detected in the genera Rhodoferax, Azospirillum, Lautropia, and Bdellovibrio and likely act as an N2O sink. A novel 15N tracer method was adapted to individually quantify N2O production and N2O consumption rates. The estimated true N2O production rate and true N2O consumption rate were 3.98 ± 0.15 and 3.03 ± 0.18 mgN·gVSS–1·day–1, respectively. The N2O consumption rate could be increased by 51% (4.57 ± 0.51 mgN·gVSS–1·day–1) with elevated N2O concentrations but kept comparable irrespective of the presence or absence of NO2. Collectively, the approach allowed the quantification of N2O-reducing activity and the identification of transcriptionally active N2O reducers that may constitute as an N2O sink in anammox-based processes.

中文翻译:

的组合15 Ñ示踪和微生物分析公开了N- 2的厌氧氨氧化社区的O-水槽潜在

尽管通过部分亚硝化和厌氧氨氧化脱氮比传统的硝化和反硝化更具成本效益,但一个缺点是一氧化二氮 (N 2 O)的产生和积累。N 2 O 还原细菌(厌氧氨氧化微生物群落的常驻成员)用于 N 2 O 缓解的潜在开发需要对其生态生理学有更多了解。本研究调查了厌氧氨氧化微生物群落中N 2 O 还原菌的系统发育,并单独量化了 N 2 O 生产和 N 2 O 消耗的过程。上流式塔床厌氧氨氧化反应器,用 NH 4 +和 NO进料2 没有氧气,以 0.284%的平均转化率(产生的 N 2 O:流入的氮)排放 N 2 O。反应器生物质中具有转录活性且高度丰富的nosZ基因属于伯克霍尔德菌科(进化枝 I 型)和绿屈挠属(进化枝 II 型)。同时,较不丰富的而是积极转录nosZ在属中检测菌株Rhodoferax固氮螺菌属Lautropia,和蛭弧菌和可能充当一个N 2 O灌。一本小说15N 示踪剂方法适用于单独量化 N 2 O 生产和 N 2 O 消耗率。估计的真实 N 2 O 生成率和真实 N 2 O 消耗率分别为 3.98 ± 0.15 和 3.03 ± 0.18 mg N ·g VSS –1 ·day –1。随着 N 2 O 浓度升高,N 2 O 消耗率可增加 51%(4.57 ± 0.51 mg N ·g VSS –1 ·day –1),但无论是否存在 NO 2 – 都保持可比性. 总的来说,该方法允许量化 N 2 O 还原活性并鉴定转录活性 N 2 O 还原剂,它们可能构成基于厌氧氨氧化的过程中的 N 2 O 汇。
更新日期:2021-07-06
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