Journal of Histochemistry & Cytochemistry ( IF 3.2 ) Pub Date : 2021-06-18 , DOI: 10.1369/00221554211025327 Ida Biunno 1, 2 , Emanuela Paiola 3 , Pasquale De Blasio 1
“Multi-Omics” technologies have contributed greatly to the understanding of various diseases by enabling researchers to accurately and rapidly investigate the molecular circuitry that connects cellular systems. The tissue-engineered, three-dimensional (3D), in vitro disease model “organoid” integrates the “omics” results in a model system, elucidating the complex links between genotype and phenotype. These 3D structures have been used to model cancer, infectious disease, toxicity, and neurological disorders. Here, we describe the advantage of using the tissue microarray (TMA) technology to analyze human-induced pluripotent stem cell–derived cerebral organoids. Compared with the conventional processing of individual samples, sectioning and staining of TMA slides are faster and can be automated, decreasing labor and reagent costs. The TMA technology faithfully captures cell morphology variations and detects specific biomarkers. The use of this technology can scale up organoid research results in at least two ways: (1) in the number of specimens that can be analyzed simultaneously and (2) in the number of consecutive sections that can be produced for analysis with different probes and antibodies.
中文翻译:
组织微阵列 (TMA) 技术在脑类器官分析中的应用
“多组学”技术使研究人员能够准确、快速地研究连接细胞系统的分子回路,为了解各种疾病做出了巨大贡献。组织工程的三维 (3D) 体外疾病模型“类器官”将“组学”结果整合到一个模型系统中,阐明了基因型和表型之间的复杂联系。这些 3D 结构已被用于模拟癌症、传染病、毒性和神经系统疾病。在这里,我们描述了使用组织微阵列 (TMA) 技术分析人类诱导的多能干细胞衍生的大脑类器官的优势。与单个样品的常规处理相比,TMA 载玻片的切片和染色速度更快,并且可以自动化,从而降低劳动力和试剂成本。TMA 技术忠实地捕捉细胞形态变化并检测特定的生物标志物。使用该技术至少可以通过两种方式扩大类器官研究结果:(1)可以同时分析的样本数量和(2)可以产生用于使用不同探针进行分析的连续切片的数量,以及抗体。