BACKGROUND:Blackcurrants are an important berry crop whose further development depends mainly on the diversity of available plant material and its successful inclusion in the breeding. OBJECTIVE:The study aimed to evaluate selected SSR markers’ applicability for the analysis of germplasm consistingof genotypes developed through complicated multistage crosses among different Ribes species and estimate the genetic structure and level of genetic diversity of the blackcurrant collection. METHODS:The set of 110 blackcurrant accessions from the Latvian genetic resources collection was genotyped using 18 previously described SSR markers. RESULTS:The marker set provided all genotypes with unique fingerprints and proved the need for reference genotypes in international studies for data validation. The accessions clustered according to pedigree and did not group by country of origin or breeding programme. CONCLUSIONS:The tested SSR markers uncovered relationships between blackcurrant accessions of complicated interspecific composition and revealed an extensive mutual exchange of germplasm among breeding programmes, thus pointing to the need for new donors of valuable characteristics. The results also highlighted the necessity to identify each species’ proportion in the hybrid material to assess the impact of species-linked gene expression.

中文翻译：

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基于微卫星指纹图谱的黑加仑种质结构评价用于育种材料的多样化

背景：黑加仑子是一种重要的浆果作物，其进一步发展主要取决于可用植物材料的多样性及其在育种中的成功纳入。目的：本研究旨在评估选定的SSR标记在分析由不同Ribes物种之间复杂的多阶段杂交产生的基因型组成的种质的适用性，并估计黑加仑集合的遗传结构和遗传多样性水平。方法：使用 18 个先前描述的 SSR 标记对来自拉脱维亚遗传资源收藏的 110 个黑加仑种质进行基因分型。结果：标记集为所有基因型提供了独特的指纹，并证明了在国际研究中需要参考基因型进行数据验证。种质根据谱系聚类，不按原产国或育种计划分组。结论：所测试的 SSR 标记揭示了复杂种间组成的黑加仑种质之间的关系，并揭示了育种计划之间广泛的种质相互交换，从而表明需要具有有价值特征的新供体。结果还强调了确定每个物种在杂交材料中的比例以评估物种相关基因表达影响的必要性。因此指出需要具有有价值特征的新捐助者。结果还强调了确定每个物种在杂交材料中的比例以评估物种相关基因表达影响的必要性。因此指出需要具有有价值特征的新捐助者。结果还强调了确定每个物种在杂交材料中的比例以评估物种相关基因表达影响的必要性。