The combination of poorly drained soils and high rainfall can cause transient waterlogging and reduce yield of lentil. We screened 111 lentil lines for response to waterlogging in 2019 and 2020 using a pot assay outdoors. At 484 °Cd after emergence (38 d) in 2019 and 452 °Cd after emergence (42 d) in 2020, plants were waterlogged for 184 °Cd (11 d, 2019) and 167 °Cd (14 d, 2020) and allowed to recover for 323 °Cd (20 d, 2019) and 307 °Cd (26 d, 2020). We combined 2-D digital images of canopy cover and plant height to derive a 3-D trait that correlates with biomass to derive plant growth rate. Actual biomass at the end of recovery in the waterlogged plants varied 2.6-fold with genotype, and genotypic and phenotypic correlations showed associations with plant growth rate both during (r_g = .92; r_p = .74) and after waterlogging (r_g = .75; r_p = .72); there was no trade-off between maintenance of growth during waterlogging and growth during recovery. The ratio of biomass between waterlogged and control plants at the end of recovery was associated with growth rate during recovery (r_g = .88, r_p = .65) and biomass at the end of waterlogging (r_g = .61, r_p = .67). Broad-sense heritability was 0.27 for growth rate during waterlogging, 0.37 for growth rate during recovery, 0.51 for biomass at the end of waterlogging and 0.47 for biomass at the end of recovery. High biomass at the end of recovery correlated with cooler canopies but correlations varied with season and measurement date, and heritability of canopy temperature was low. We identified genotypes with consistently higher tolerance to waterlogging and provide an improved understanding of the physiological response of lentil to hypoxia highlighting the importance of growth rate not only during waterlogging but also during recovery.

中文翻译：

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植物生长速率的高通量表型筛选小扁豆耐涝性

排水不良的土壤和高降雨量的结合会导致短暂的内涝并降低小扁豆的产量。我们在 2019 年和 2020 年使用户外盆栽试验筛选了 111 条小扁豆品系对涝渍的反应。在 2019 年出苗后（38 天）和 2020 年出苗后（42 天）484 °Cd 下，植物浸水 184 °Cd（2019 年第 11 天）和 167 °Cd（2020 年第 14 天）并允许恢复 323 °Cd（20 天，2019 年）和 307 °Cd（26 天，2020 年）。我们结合冠层盖度和植物高度的 2-D 数字图像来推导出与生物量相关的 3-D 性状，以推导出植物生长速率。浸水植物恢复结束时的实际生物量随基因型变化 2.6 倍，基因型和表型相关性显示与植物生长速率相关 ( r _g  = .92;r _p  = .74) 和淹水后 ( r _g  = .75; r _p  = .72); 在淹水期间保持生长和恢复期间的生长之间没有权衡。恢复结束时淹水植物和对照植物之间的生物量比与恢复期间的生长速率 ( r _g  = .88, r _p  = .65) 和淹水结束时的生物量 ( r _g  = .61, r _p = .67）。广义遗传力为涝期生长速率0.27，恢复期生长速率0.37，涝期生物量0.51，恢复期生物量0.47。恢复结束时的高生物量与较冷的冠层相关，但相关性随季节和测量日期而变化，冠层温度的遗传力较低。我们确定了对涝渍具有持续较高耐受性的基因型，并提供了对扁豆对缺氧的生理反应的更好理解，突出了不仅在涝渍期间而且在恢复期间生长速率的重要性。