High-level mupirocin resistance (HLMR) is determined by the plasmid-located ileS2 gene flanked by two copies of the insertion sequence 257 (IS257). The molecular epidemiology of high-level mupirocin-resistant isolates could be assessed by the determination of their IS257-ileS2 spacer regions conformation. In this study, 188 isolates of methicillin-resistant staphylococci were subjected to the detection of HLMR, and analysis of the conformation of the IS257-ileS2 spacer regions. Mupirocin resistance was detected in five (2,6%) isolates, among which two were recognized as Staphylococcus pseudintermedius, two as Staphylococcus haemolyticus, and one as Staphylococcus aureus. High-level mupirocin resistance was revealed by the agar disk diffusion method, and MIC values, and was confirmed by the detection of the ileS2 gene. The conformations of the IS257-ileS2 spacer regions were homologous in two S. haemolyticus strains tested. The remaining three isolates showed diverse IS257-ileS2 conformations. The results of this study indicate that HLMR occasionally occurs in staphylococci isolated from companion animals. The heterogeneity and the homogeneity of the IS257-ileS2 spacer regions confirm that the ileS2 gene spread among staphylococci of animal origin by the transfer of different as well as the same plasmids. Surveillance of the occurrence of mupirocin resistance and molecular characterization of resistant isolates are strongly recommended due to the possibility of plasmid-located resistance gene transfer between staphylococci.

高水平莫匹罗星抗性 (HLMR) 由位于质粒的ileS2基因决定，该基因两侧是插入序列 257 (IS257) 的两个拷贝。高水平抗莫匹罗星分离株的分子流行病学可以通过测定它们的 IS257- ileS2间隔区构象来评估。本研究对188株耐甲氧西林葡萄球菌进行HLMR检测和IS257- ileS2间隔区构象分析。在 5 株（2.6%）分离株中检测到莫匹罗星耐药，其中 2 株为假中间葡萄球菌，2株为溶血葡萄球菌， 1株为金黄色葡萄球菌. 高水平的莫匹罗星耐药性通过琼脂盘扩散法和 MIC 值显示，并通过ileS2基因的检测得到证实。IS257- ileS2间隔区的构象在两个测试的溶血链球菌菌株中是同源的。其余三个分离株显示出不同的 IS257- ileS2构象。这项研究的结果表明，HLMR 偶尔会发生在从伴侣动物中分离出来的葡萄球菌中。IS257- ileS2间隔区的异质性和同质性证实了ileS2基因通过不同和相同质粒的转移在动物源葡萄球菌中传播。由于葡萄球菌之间存在质粒定位抗性基因转移的可能性，强烈建议监测莫匹罗星抗性的发生和抗性分离株的分子特征。