Probiotics development for animal farming implies thorough testing of a vast variety of properties, including adhesion, toxicity, host cells signaling modulation, and immune effects. Being diverse, these properties are often tested individually and using separate biological models, with great emphasis on the host organism. Although being precise, this approach is cost-ineffective, limits the probiotics screening throughput and lacks informativeness due to the ‘one model - one test - one property’ principle. There is а solution coming from human-derived cells and in vitro systems, an extraordinary example of human models serving animal research. In the present review, we focus on the current outlooks of employing human-derived in vitro biological models in probiotics development for animal applications, examples of such studies and the analysis of concordance between these models and host-derived in vivo data. In our opinion, human-cells derived screening systems allow to test several probiotic properties at once with reasonable precision, great informativeness and less expenses and labor effort.

用于动物养殖的益生菌开发意味着对各种特性进行彻底测试，包括粘附、毒性、宿主细胞信号调节和免疫效应。由于是多样化的，这些特性通常单独进行测试，并使用单独的生物模型进行测试，重点放在宿主生物体上。尽管精确，但这种方法成本效益低，限制了益生菌筛选通量，并且由于“一个模型-一个测试-一个属性”的原则而缺乏信息量。有一种来自人源细胞和体外系统的解决方案，这是为动物研究服务的人类模型的一个非凡例子。在本综述中，我们重点关注在体外使用人源性用于动物应用的益生菌开发中的生物模型、此类研究的示例以及这些模型与宿主衍生的体内数据之间的一致性分析。在我们看来，源自人体细胞的筛选系统允许以合理的精度、丰富的信息量和更少的费用和劳动力同时测试多种益生菌特性。