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The Use of Ultraviolet Light as a Non-destructive Method for Revealing Fragments of Lost Pigmentation in Faded Alcohol-preserved Collection Specimens
Proceedings of the Academy of Natural Sciences of Philadelphia ( IF 0.7 ) Pub Date : 2016-11-01 , DOI: 10.1635/053.165.0112
Andre L. Netto-Ferreira 1 , Kyle R. Luckenbill 2
Affiliation  

ABSTRACT. A method for visualizing previously pigmented areas of the body with the employment of black-light spectrum bulbs is described. Under ultraviolet (UV) light, fish musculature fluoresces, whereas melanophores absorb incident light, revealing nearly lost contrast between once strongly and poorly pigmented areas of the body that has faded over time. Although a yet unknown extent of melanophore preservation is necessary to expose such contrast, the method was successful on a partially faded holotype of Characidium fasciadorsale Fowler, a nearly completely faded holotype of Characidium etheostoma Cope, and most remarkably for the blackened, previously mold-infested and nearly destroyed holotype of Holotaxis laetus Cope. In the latter case, the method also allowed better recognition of scale pockets on the skin.

中文翻译:

使用紫外线作为一种无损方法来揭示褪色的酒精保存收藏标本中丢失的色素碎片

摘要。描述了一种使用黑光谱灯泡可视化先前着色的身体区域的方法。在紫外线 (UV) 光下,鱼的肌肉组织会发出荧光,而黑色素细胞会吸收入射光,这表明身体曾经随着时间推移而褪色的强烈和暗淡区域之间几乎失去了对比度。尽管需要未知程度的黑色素细胞保存才能暴露这种对比,但该方法在部分褪色的 Characidium fasciadorsale Fowler 正模标本、近乎完全褪色的 Characidium etheostoma Cope 正模标本上取得了成功,最显着的是对于已发黑、以前被霉菌侵染的黑素细胞和几乎毁坏的 Holotaxi laetus Cope 正模。在后一种情况下,该方法还可以更好地识别皮肤上的水垢袋。
更新日期:2016-11-01
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