A potentially scalable configuration of a H₂-based MBfR was developed in this study to test the feasibility of treating real secondary effluent. Synthetic water was first fed for biofilm attachment, and afterwards, the influent was switched to real secondary effluent once efficient denitrification was achieved. The biofilm reduced 15 mg N per L nitrate to 5 mg N per L with a maximum nitrate removal flux of 1.0 g N per m² d. Kinetic parameters of q_max and K were also estimated to be 0.08 mg NO₃⁻-N mg per VS d and 0.56 mg NO₃⁻-N per L, respectively, using the finite difference method. Spearman's correlation analysis showed that the microbial community diversity increased as sulfate was introduced in real secondary effluent. Desulfomicrobium and Desulfotomaculum were most influenced by SO₄²⁻ loading among dominant bacteria. Cost assessment demonstrated that this MBfR system showed both lower operation cost (0.67 CNY per t) and smaller footprint (0.20 m² t⁻¹ d⁻¹), thus demonstrating considerable advantages over other advanced nitrogen removal technologies.

中文翻译：

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基于 H2 的 MBfR 在实际市政二级出水深度脱氮中的应用

在这项研究中开发了一种基于H ₂的 MBfR的潜在可扩展配置，以测试处理真正的二次流出物的可行性。合成水首先用于生物膜附着，然后，一旦实现有效的反硝化，进水就切换到真正的二级出水。生物膜将每升硝酸盐 15 毫克 N 减少到每升 5 毫克 N，最大硝酸盐去除通量为每米²天1.0 克 N。q _max和K 的动力学参数也估计为0.08 mg NO ₃ ^- -N mg/VS d 和0.56 mg NO ₃ ^--N per L，分别使用有限差分法。Spearman 的相关性分析表明，随着硫酸盐的引入，微生物群落多样性增加。Desulfomicrobium和Desulfotomaculum在优势细菌中受 SO ₄ ^2-负荷的影响最大。成本评估表明，该 MBfR 系统显示出较低的运行成本（每吨 0.67 元人民币）和较小的占地面积（0.20 m ² t ^-1 d ^-1），因此与其他先进的脱氮技术相比具有相当大的优势。