A steady supply of platelets maintains their levels in the blood, and this is achieved by the generation of progeny from platelet intermediates. Using systematic super-resolution microscopy, we examine the ultrastructural organization of various organelles in different platelet intermediates to understand the mechanism of organelle redistribution and sorting in platelet intermediate maturation as the early step of platelet progeny production. We observe the dynamic interconversion between the intermediates and find that microtubules are responsible for controlling the overall shape of platelet intermediates. Super-resolution images show that most of the organelles are located near the cell periphery in oval preplatelets and confined to the bulbous tips in proplatelets. We also find that the distribution of the dense tubular system and α granules is regulated by actin, whereas that of mitochondria and dense granules is governed by microtubules. Altogether, our results call for a reassessment of organelle redistribution in platelet intermediates.

血小板的稳定供应维持其在血液中的水平，这是通过从血小板中间体产生后代来实现的。我们使用系统的超分辨率显微镜检查不同血小板中间体中各种细胞器的超微结构组织，以了解作为血小板后代产生的早期步骤的血小板中间体成熟中细胞器重新分布和分选的机制。我们观察中间体之间的动态相互转换，发现微管负责控制血小板中间体的整体形状。超分辨率图像显示，大多数细胞器位于椭圆形前血小板中的细胞周边附近，并且局限于前血小板中的球状尖端。我们还发现致密管状系统和α颗粒的分布受肌动蛋白调节，而线粒体和致密颗粒的分布受微管控制。总之，我们的结果要求重新评估血小板中间体中细胞器的重新分布。