Lipopolysaccharides (endotoxins), found on Gram-negative bacteria, can trigger a severe immune response in humans leading to septic shock and in extreme cases, even death. Therefore, the detection and neutralization of lipopolysaccharides (LPS) is of utmost importance in the pharmaceutical and medical industries. The United States Food and Drug Administration (US FDA) recommended detection method for LPS, the Limulus amebocyte lysate (LAL) assay, is expensive, time consuming, complex, and is prone to interference from proteases. As an alternative, this paper proposes a rapid, label-free fluorescence-based assay using LPS-specific aptamers and the SYBR Green DNA stain. The proposed method has a detection limit of 0.1 ng/ml, which is sufficient to detect the permissible levels of LPS in many pharmaceutical drugs and medical products. The fluorescence signal was found to be a linear function of the concentration of LPS in the range from 0.1 ng/ml to 10⁵ ng/ml.

中文翻译：

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使用 SYBR Green I 快速检测脂多糖的基于适体的荧光检测

在革兰氏阴性菌中发现的脂多糖（内毒素）可引发人类严重的免疫反应，导致感染性休克，在极端情况下甚至导致死亡。因此，脂多糖 (LPS) 的检测和中和在制药和医疗行业中至关重要。美国食品和药物管理局 (US FDA) 推荐的 LPS 检测方法，即鲎变形细胞裂解物 (LAL) 测定法，昂贵、耗时、复杂且容易受到蛋白酶的干扰。作为替代方案，本文提出了一种使用 LPS 特异性适体和 SYBR Green DNA 染色剂的快速、无标记荧光检测。所提出的方法的检测限为 0.1 ng/ml，足以检测许多药物和医疗产品中 LPS 的允许水平。⁵纳克/毫升。