In this study, marine biofilms were cultured in a flow-lane, semi-continuous photobioreactor at different irradiances and flows to evaluate their combined effect on biofilms’ phototrophic composition and photosynthetic activity. Taxon richness, evaluated by different microscopy techniques, including transmission and scanning electron microscopy, resulted to be heavily reduced from source communities to mature cultures. The strongest decrease was observed for diatoms, which were overcome by cyanobacteria and green algae over time. Photosynthetic performance was investigated by pulse amplitude modulated fluorescence. Irradiance was the main driver of data distribution of the photosynthetic parameters rel.ETR_max and I_k, while flow rate affected α and ΔF/F_m’. The combination of irradiance and flow rate affected ΔF/F_m’ reflecting the photosynthetic performance of the most relatively abundant taxa. Higher ΔF/F_m’ was attained when cyanobacteria and green algae were dominating, whilst lower ΔF/F_m’ when diatoms occurred in the initial phase of biofilm development.

在这项研究中，海洋生物膜在流道、半连续光生物反应器中以不同的辐照度和流量培养，以评估它们对生物膜光合组成和光合活性的综合影响。通过不同的显微镜技术（包括透射和扫描电子显微镜）评估的分类群丰富度导致从源群落到成熟文化的严重减少。观察到硅藻的减少最强烈，随着时间的推移，它们被蓝藻和绿藻所克服。通过脉冲幅度调制荧光研究光合性能。辐照度是光合参数rel.ETR _max数据分布的主要驱动因素和 I _k，而流速影响α 和 ΔF/F _m '。辐照度和流速的组合影响了 ΔF/F _m '，反映了最丰富的分类群的光合性能。较高的ΔF/ F_米“被获得当蓝藻和绿藻被支配，而较低的ΔF/ F_米”时，硅藻发生在生物膜发展的初始阶段。