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Effect of planting method on C:N:P stoichiometry in soils, young silver fir (Abies alba Mill.) and stone pine (Pinus cembra L.) in the upper mountain zone of karpaty mountains
Ecological Indicators ( IF 6.9 ) Pub Date : 2021-06-17 , DOI: 10.1016/j.ecolind.2021.107905
Jarosław Lasota , Stanisław Małek , Michał Jasik , Ewa Błońska

C/N/P stoichiometry offers important indicators of ecosystem function and productivity. The aim of this research was to determine how planting methods in biogroups influence C/N/P stoichiometry in soil and plants. We hypothesised that planting methods, consisting in different initial spacing and shape would affect the growth characteristics of trees, and consequently, change soil properties – microbiological activity, in particular. This research examined two species that have different ecological requirements, silver fir (Abies alba Mill.) and Swiss stone pine (Pinus cembra L.), grown in the upper mountain zone, 1200 m above sea level. We established 48 experimental plots with two planting methods and two species in 2011. The soil and plant samples for laboratory analysis were taken in September 2020. In the first planting method 49 seedlings at regular 1 × 1 m spacing in a square plot (PM1) were planted, while in the second method 50 seedlings in two rhombus plots with 50 × 50 cm spacing (PM2) were planted. We determined the basic physicochemical properties and enzymatic activity in soil samples, and we recorded the content of C, N and P in testing needle samples from the trees. We also calculated the C/N, C/P and N/P ratios for both the soil samples and the plants. We found that the formation of biogroups with smaller spacing during stand renewal influenced the selected soil properties; within the biogroups with smaller spacing, an increase in soil biochemical activity was found. Our research confirms that planting methods and species are essential contributors to the C/N/P stoichiometry of soils and plants. The C/N/P stoichiometry of the soil samples from the different species and the different planting methods ranged between 928/37/1 and 1087/42/1. In the case of the Swiss stone pine needles, we noted a narrower C/N/P stoichiometry compared to the fir needles, regardless of the planting method. C/N/P stoichiometry is a useful tool for reflecting the nutrient cycle in mountain ecosystems, and our research shows that in the case of species with higher ecological requirements, stoichiometry can indicate nutrient limitation. In higher mountainous locations, nitrogen and phosphorus deficiencies are the main limiting factor. In the case of fir trees, the method of planting helps to reduce nutrient limitation and improve growth characteristics.



中文翻译:

种植方式对卡帕蒂山上山区土壤、幼银冷杉(Abies alba Mill.)和石松(Pinus cembra L.)C:N:P化学计量的影响

C/N/P 化学计量提供了生态系统功能和生产力的重要指标。本研究的目的是确定生物群中的种植方法如何影响土壤和植物中的 C/N/P 化学计量。我们假设由不同的初始间距和形状组成的种植方法会影响树木的生长特性,从而改变土壤特性——尤其是微生物活动。这项研究检查了两种具有不同生态要求的物种,即银杉 ( Abies alba Mill.) 和瑞士石松 ( Pinus cembraL.),生长在海拔 1200 m 的高山地带。2011 年我们建立了 48 个试验小区,两种种植方式和两个物种。用于实验室分析的土壤和植物样品于 2020 年 9 月采集。在第一种种植方式中,49 株幼苗以 1 × 1 m 的规则间距在方形小区(PM1)中种植,而在第二种方法中,在两个间距为 50 × 50 厘米 (PM2) 的菱形地块中种植了 50 株幼苗。我们确定了土壤样品中的基本理化性质和酶活性,并记录了测试树木针样品中 C、N 和 P 的含量。我们还计算了土壤样品和植物的 C/N、C/P 和 N/P 比率。我们发现林分更新过程中形成的间距较小的生物群会影响选定的土壤性质;在间距较小的生物群中,发现土壤生化活性增加。我们的研究证实,种植方法和物种是土壤和植物 C/N/P 化学计量的重要贡献者。来自不同物种和不同种植方法的土壤样品的 C/N/P 化学计量介于 928/37/1 和 1087/42/1 之间。就瑞士石松针而言,无论种植方法如何,我们都注意到与冷杉针相比,C/N/P 化学计量更窄。C/N/P 化学计量是反映山区生态系统养分循环的有用工具,我们的研究表明,对于生态需求较高的物种,化学计量可以表明养分限制。在较高的山区,氮和磷的缺乏是​​主要的限制因素。

更新日期:2021-06-17
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