Immobilization of Escherichia coli (E. coli) on commercial activated charcoal was enhanced by mild chemical treatment coupled with curli production from E. coli. The chemical used to treat the activated charcoal were sodium hydroxide, hydrochloric acid, ammonium hydroxide, and acetic acid while nickel (II) chloride was used to promote the production of curli. Characteristics of the activated charcoal before and after chemical treatments were analyzed including its surface properties, pore size, and crystalline structure. The immobilization of E. coli was enhanced greatly after sodium hydroxide treatment which gave rise to more than 120 % cell immobilized compared to the untreated activated charcoal which was mainly attributed to the larger size of macropore, surface area, and pore volume. Curli were produced by the induction of nickel (II) chloride and further enhanced the cell immobilization by at least 50 %. Overall, the combine strategy enhanced cell immobilization by more than 160 %. The resulting biocatalyst from the enhanced cell immobilization managed to be reused up to 10 cycles for the enzyme cyclodextrin glucanotransferase expression while retaining up to 60 % of the enzyme’s initial activity.

的固定化大肠杆菌（大肠杆菌商业活性炭）通过加上从curli生产温和的化学处理来提高大肠杆菌。用于处理活性炭的化学品是氢氧化钠、盐酸、氢氧化铵和乙酸，而氯化镍 (II) 用于促进卷曲的产生。分析了化学处理前后活性炭的特性，包括其表面性质、孔径和晶体结构。大肠杆菌的固定化与未处理的活性炭相比，氢氧化钠处理后细胞固定化率大大提高，这主要归因于大孔、表面积和孔体积的更大尺寸。Curli 是通过诱导氯化镍 (II) 产生的，并进一步将细胞固定化提高了至少 50%。总体而言，组合策略将细胞固定化提高了 160% 以上。增强的细胞固定化产生的生物催化剂能够重复使用多达 10 个循环，用于环糊精葡聚糖转移酶的表达，同时保留了酶初始活性的 60%。