Flesh browning (FB) of ‘Cripps Pink’ apples can cause significant economic losses to apple producers worldwide. FB symptoms and etiology can vary for this cultivar. Specific metabolic fingerprint has the potential for identification and understanding of different disorders leading FB postharvest. Flesh tissue from fruit collected starting 150 days after full bloom, through harvest, then, until 7 months into cold storage were sampled for non-targeted metabolic analyses using gas and liquid chromatography coupled with mass spectrometry. Radial FB development was positively associated with sphingolipids metabolism. Elevated levels of multiple glucocerebrosides and ceramides were found in apples at risk for developing the disorder and with symptoms, while the concentrations of a different group of inter-related metabolites tentatively as sphingolipids increased in healthy tissue during storage. Based on previous reports in other plant and animal species, elevated sphingolipid concentrations may be associated with membrane lipid catabolism, cell apoptosis, and fruit senescence involved in FB symptom development.

'Cripps Pink' 苹果的果肉褐变 (FB) 会对全球苹果生产商造成重大经济损失。该品种的 FB 症状和病因可能有所不同。特定的代谢指纹具有识别和理解导致 FB 收获后的不同疾病的潜力。从盛开后 150 天开始收集水果的肉组织，直到收获，然后直到冷藏 7 个月，使用气相和液相色谱结合质谱法对非靶向代谢分析进行采样。径向 FB 发育与鞘脂代谢呈正相关。在有患病风险和有症状的苹果中发现了多种葡糖脑苷脂和神经酰胺水平升高，而另一组相互关联的代谢物在储存期间在健康组织中的浓度暂时增加。根据之前在其他植物和动物物种中的报告，鞘脂浓度升高可能与膜脂分解代谢、细胞凋亡和参与 FB 症状发展的果实衰老有关。