Human papillomavirus (HPV) has more than 100 different types, some of which are associated with cancer. The most common example is that of cervical cancer, which is associated with HPV16 and HPV18. Here, we performed next-generation sequencing (NGS) to type 2436 samples obtained from Korean women to elucidate the correlation between multiple infections, virus types, and cytology. NGS revealed that types 58, 56, and 16 were the most common in high-risk (HR) types, whereas types 90, 54, and 81 were the most common in low-risk (LR) types. The incidence of atypical squamous cells of undetermined significance (ASCUS) or high-grade squamous intraepithelial lesion (HSIL) was 11.45% in single-type cases and 27.17% in multiple infections by the two types of HPV. ASCUS or HSIL was 29.79% in only the HR type multiple infections and 29.81% in mixed high- and low-risk types of multiple infections, whereas it was 18.79% in LR type multiple infections (P ≤ 0.0001). Co-infection by LR-HPV and HR-HPV is therefore more likely to cause cell lesions. Collectively, these results show that the higher the incidence of multiple infections, the greater the frequency of cell lesions. Thus, to predict the clinical symptoms, it would be beneficial to confirm the HPV type and multiple infections using NGS, although this could be relatively expensive.

中文翻译：

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用于分型人乳头瘤病毒和预测多重感染及其临床症状的下一代测序

人乳头瘤病毒 (HPV) 有 100 多种不同类型，其中一些与癌症有关。最常见的例子是宫颈癌，它与 HPV16 和 HPV18 相关。在这里，我们对从韩国女性获得的 2436 份样本进行了新一代测序 (NGS)，以阐明多种感染、病毒类型和细胞学之间的相关性。NGS 显示 58、56 和 16 型在高风险 (HR) 类型中最常见，而 90、54 和 81 型在低风险 (LR) 类型中最常见。两种类型 HPV 感染的单型病例中非典型鳞状细胞（ASCUS）或高级别鳞状上皮内病变（HSIL）的发生率为 11.45% 和 27.17%。ASCUS 或 HSIL 仅在 HR 型多重感染中为 29.79%，在 29.P  ≤0.0001）。因此，LR-HPV 和 HR-HPV 共同感染更可能导致细胞损伤。总的来说，这些结果表明多重感染的发生率越高，细胞病变的频率就越高。因此，为了预测临床症状，使用 NGS 确认 HPV 类型和多重感染将是有益的，尽管这可能相对昂贵。