Microscopic examination results in patients with filariasis are often not identified by the presence of microfilaria, so it needs to be checked by Polymerase Chain Reaction (PCR). One PCR method uses High-Resolution Melting (HRM). The purpose of this study was to compare qPCR-HRM with microscopic examination methods to determine the types of microfilaria found in patients with filariasis. 19 samples were examined using a microscopic method and qPCR-HRM. The results of microscopic examination found no type of microfilaria and in qPCR-HRM identified B.malayi and W.bancrofti with peak temperature melting 78.1–78.7 ℃ and 80.2–80.8 ℃. The results of the study based on the comparison of two methods show that the types of microfilaria W.bancrofti and B.malayi can be found using qPCR-HRM.

丝虫病患者的显微镜检查结果往往不能通过微丝蚴的存在来识别，因此需要通过聚合酶链反应（PCR）进行检查。一种 PCR 方法使用高分辨率熔解 (HRM)。本研究的目的是将 qPCR-HRM 与显微镜检查方法进行比较，以确定丝虫病患者中发现的微丝蚴类型。使用显微镜方法和 qPCR-HRM 检查了 19 个样品。显微镜检查结果未发现微丝蚴类型，qPCR-HRM 鉴定出马来蚴和班氏蚴，其熔点峰值温度分别为78.1-78.7 ℃和80.2-80.8 ℃。基于两种方法比较的研究结果表明，微丝蚴的类型W.bancrofti和B.malayi可以使用 qPCR-HRM 找到。