Pineapple plant (Ananas comosus) is one of the largest productions in Asia and its increasing production has generated a huge amount of pineapple wastes. Pineapple plant stem is made up of high concentration of starch which can potentially be converted into value-added products, including amino acids. Due to the increasing demand in animal feed grade amino acids, especially for methionine and lysine, the utilisation of cheap and renewable source is deemed to be an essential approach. This study aimed to produce amino acids from pineapple plant stem hydrolysates through microbial fermentation by Pediococcus acidilactici Kp10. Dextrozyme was used for hydrolysis of starch and Celluclast 1.5 L for saccharification of cellulosic materials in pineapple plant stem. The hydrolysates obtained were used in the fermentation to produce methionine and lysine. Pineapple plant stem showed high starch content of 77.78%. Lignocellulosic composition of pineapple plant stem consisted of 46.15% hemicellulose, 31.86% cellulose, and 18.60% lignin. Saccharification of alkaline-treated pineapple plant stem gave lower reducing sugars of 13.28 g/L as compared to untreated, where 18.56 g/L reducing sugars obtained. Therefore, the untreated pineapple plant stem was selected for further process. Starch hydrolysis produced 57.57 g/L reducing sugar (100% hydrolysis yield) and saccharification of cellulosic materials produced 24.67 g/L reducing sugars (56.93% hydrolysis yield). The starch-based and cellulosic-based of pineapple plant stem were subjected as carbon source in methionine and lysine production by P. acidilactici Kp10. In conclusion, higher methionine and lysine production were produced from starch-based hydrolysis (40.25 mg/L and 0.97 g/L, respectively) as compared to cellulosic-based saccharification (37.31 mg/L and 0.84 g/L, respectively) of pineapple plant stem.

中文翻译：

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从菠萝（Ananas comosus）植物茎中提取的淀粉作为氨基酸生产的来源

菠萝植物（Ananas comosus）是亚洲最大的生产地之一，其不断增加的产量产生了大量的菠萝废料。菠萝植物茎由高浓度淀粉组成，可以转化为增值产品，包括氨基酸。由于对动物饲料级氨基酸的需求不断增加，尤其是蛋氨酸和赖氨酸，因此利用廉价和可再生资源被认为是必不可少的方法。本研究旨在通过乳酸片球菌 Kp10 的微生物发酵从菠萝植物茎水解物中生产氨基酸。Dextrozyme 用于淀粉水解，Celluclast 1.5 L 用于菠萝植物茎中纤维素材料的糖化。获得的水解产物用于发酵以生产甲硫氨酸和赖氨酸。菠萝植株茎中淀粉含量高达77.78%。菠萝植物茎的木质纤维素成分由 46.15% 的半纤维素、31.86% 的纤维素和 18.60% 的木质素组成。与未处理的菠萝植物茎相比，碱处理的菠萝植物茎的糖化产生较低的还原糖 13.28 g/L，其中获得 18.56 g/L 的还原糖。因此，选择未经处理的菠萝植物茎进行进一步加工。淀粉水解产生 57.57 g/L 还原糖（100% 水解产率），纤维素材料的糖化产生 24.67 g/L 还原糖（56.93% 水解产率）。菠萝植株茎的淀粉基和纤维素基作为碳源在 P. acidilactici Kp10 生产蛋氨酸和赖氨酸中进行。综上所述，