Considering the cases of fungal resistance to classic antifungals, it is necessary to develop more efficient and innovative therapies capable of reversing this situation. Fluconazole is an antifungal frequently used in the treatment of mycosis and some fungi developed resistance to its mechanism of action. In this work, fluconazole and green propolis were co-encapsulated in chitosan nanoparticles to be explored in order to promote a synergistic effect to enhance its therapeutic efficacy. However, because of the complexity of the chemical composition of green propolis, it was necessary to develop a simple and precise methodology to quantify fluconazole in the formulation. High Efficiency Liquid Chromatography methodology was developed and validated following the Brazilian regulatory guidelines (ANVISA, RDC 166/2017) for the separation of co-eluted peaks of fluconazole and green propolis in the nanoparticle supernatant. Applying the method developed, it was possible to quantify fluconazole in the same sample containing propolis. Thus, the results allow to affirm that it is a specific test, effective, precise and robust, which helped to determine the efficiency of association of the compounds within the nanoparticle. The method can be applied to quantify compounds that have similar chromatographic retention times.

考虑到真菌对经典抗真菌药物产生耐药性的情况，有必要开发更有效和创新的疗法来扭转这种情况。氟康唑是一种常用于治疗真菌病的抗真菌剂，一些真菌对其作用机制产生抗药性。在这项工作中，将氟康唑和绿蜂胶共同包裹在壳聚糖纳米颗粒中进行探索，以促进协同作用以增强其治疗效果。然而，由于绿色蜂胶化学成分的复杂性，有必要开发一种简单而精确的方法来量化配方中的氟康唑。高效液相色谱方法是按照巴西监管指南（ANVISA，RDC 166/2017) 用于分离纳米颗粒上清液中氟康唑和绿蜂胶的共洗脱峰。应用所开发的方法，可以对含有蜂胶的同一样品中的氟康唑进行量化。因此，结果可以确认它是一种特定的测试，有效、精确和稳健，有助于确定纳米颗粒内化合物的缔合效率。该方法可用于量化具有相似色谱保留时间的化合物。这有助于确定纳米颗粒内化合物的缔合效率。该方法可用于量化具有相似色谱保留时间的化合物。这有助于确定纳米颗粒内化合物的缔合效率。该方法可用于量化具有相似色谱保留时间的化合物。