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Differential Expression Study of Lysine Crotonylation and Proteome for Chronic Obstructive Pulmonary Disease Combined with Type II Respiratory Failure
Canadian Respiratory Journal ( IF 2.2 ) Pub Date : 2021-06-16 , DOI: 10.1155/2021/6652297
Qing Gan 1 , Donge Tang 2 , Qiang Yan 3 , Jiejing Chen 1 , Yong Xu 2 , Wen Xue 1 , Lu Xiao 4 , Fengping Zheng 2 , Huixuan Xu 2 , Yingyun Fu 4 , Yong Dai 1, 2
Affiliation  

Introduction. The modification of lysine crotonylation (Kcr) is another biological function of histone in addition to modification of lysine acetylation (Kac), which may play a specific regulatory role in diseases. Objectives. This study compared the expression levels of Kcr and proteome between patients with chronic obstructive pulmonary disease (COPD) combined with type II respiratory failure (RF) to study the relationship between Kcr, proteome, and COPD. Methods. We tested the Kcr and proteome of COPD combined with type II RF and normal control (NC) using croton acylation enrichment technology and liquid chromatography tandem mass spectrometry (LC-MS/MS) with high resolution. Results. We found that 32 sites of 23 proteins were upregulated and 914 sites of 295 proteins were downregulated. We performed Kyoto Encyclopedia of Genes and Genomes (KEGG), protein domain, and Gene Ontology (GO) analysis on crotonylated protein. In proteomics research, we found that 190 proteins were upregulated and 151 proteins were downregulated. Among them, 90 proteins were both modified by differentially expressed crotonylation sites and differentially expressed in COPD combined with type II RF and NC. Conclusion. Differentially expressed crotonylation sites may be involved in the development of COPD combined with type II RF. 90 proteins modified by crotonylation and differentially expressed in COPD combined with type II RF can be used as markers for the study of the molecular pathogenesis of COPD combined with type II RF.

中文翻译:

慢性阻塞性肺疾病合并Ⅱ型呼吸衰竭赖氨酸巴豆酰化及蛋白质组差异表达研究

介绍。赖氨酸巴豆酰化 (Kcr) 的修饰是组蛋白除赖氨酸乙酰化 (Kac) 修饰之外的另一种生物学功能,可能在疾病中发挥特定的调节作用。目标。本研究通过比较慢性阻塞性肺疾病(COPD)合并II型呼吸衰竭(RF)患者之间Kcr和蛋白质组的表达水平,研究Kcr、蛋白质组和COPD之间的关系。方法。我们使用巴豆酰化富集技术和高分辨率液相色谱串联质谱 (LC-MS/MS) 测试了 COPD 的 Kcr 和蛋白质组结合 II 型 RF 和正常对照 (NC)。结果. 我们发现 23 种蛋白质的 32 个位点被上调,295 种蛋白质的 914 个位点被下调。我们对巴豆酰化蛋白进行了京都基因和基因组百科全书 (KEGG)、蛋白质结构域和基因本体论 (GO) 分析。在蛋白质组学研究中,我们发现有 190 种蛋白质被上调,151 种蛋白质被下调。其中,90 种蛋白质均通过差异表达的巴豆酰化位点修饰,并在 COPD 结合 II 型 RF 和 NC 中差异表达。结论. 差异表达的巴豆酰化位点可能与 COPD 合并 II 型 RF 的发展有关。COPD联合II型RF经巴豆酰化修饰差异表达的90种蛋白质可作为研究COPD联合II型RF分子发病机制的标志物。
更新日期:2021-06-16
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