当前位置: X-MOL 学术Am. J. Alzheimers Dis. Other Demen. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
MiR-130a-3p Has Protective Effects in Alzheimer’s Disease via Targeting DAPK1
American Journal of Alzheimer's Disease and other Dementias ( IF 3.4 ) Pub Date : 2021-06-15 , DOI: 10.1177/15333175211020572
Yanbo Wang 1 , Min Shi 1 , Zhenmei Hong 1 , Junling Kang 1 , Haiyan Pan 1 , Ci Yan 2
Affiliation  

The present study investigated the role and potential mechanisms of miR-130a-3p in AD. SH-SY5Y cells were treated with Aβ 1-42 to construct AD cell models. APP/PS1 mice were used for the animal experiments. MiR-130a-3p was downregulated in Aβ-induced SH-SY5Y cells. Overexpression of miR-130a-3p attenuates Aβ induced SH-SY5Y cell apoptosis. Low miR-130a-3p expression was detected in the hippocampus tissues of AD mice. The Morris water maze (MWM) results indicated that miR-130a-3p upregulation reduced the escape latency time and increased the time of AD mice spent in the target quadrant. DAPK1 was the target gene of miR-130a-3p. High DAPK1 mRNA level was detected in Aβ treated PC 12 cells and in the hippocampus tissues of AD mice. It was concluded that overexpression of miR-130a-3p may attenuate Aβ-induced neurotoxicity and improve the cognitive function of AD mice via targeting DAPK1.



中文翻译:

MiR-130a-3p 通过靶向 DAPK1 对阿尔茨海默病具有保护作用

本研究调查了 miR-130a-3p 在 AD 中的作用和潜在机制。SH-SY5Y细胞用Aβ1-42处理构建AD细胞模型。APP/PS1 小鼠用于动物实验。MiR-130a-3p 在 Aβ 诱导的 SH-SY5Y 细胞中下调。miR-130a-3p 的过表达减弱了 Aβ 诱导的 SH-SY5Y 细胞凋亡。在 AD 小鼠的海马组织中检测到低 miR-130a-3p 表达。Morris水迷宫(MWM)结果表明,miR-130a-3p上调减少了逃逸潜伏期,增加了AD小鼠在目标象限停留的时间。DAPK1 是 miR-130a-3p 的靶基因。在 Aβ 处理的 PC 12 细胞和 AD 小鼠的海马组织中检测到高 DAPK1 mRNA 水平。

更新日期:2021-06-15
down
wechat
bug