Abstract

The aim of this study was to determine the effect of vitamin E (α-Toc) and Otostgia persica leaf extract (OPLE) on the performance, immune response, and oxidative status in broiler chicken fed oxidised oil. A total of 350 one-day-old male broiler chickens were randomly attributed to seven dietary treatments. Broilers were fed diets containing fresh oil or oxidised oil without supplement, and diets containing oxidised oil supplemented with 200 mg/kg α-Toc, 100 or 200 mg/kg OPLE, 200 mg/kg α-Toc+ 100 mg/kg OPLE, and 200 mg/kg α-Toc + 200 OPLE mg/kg for six weeks. The plasma oxidative status was evaluated by measuring the superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity in plasma. Lipid peroxidation in thigh meat was measured by thiobarbituric acid-reactive substances (TBARs). Inclusion of antioxidants in the diet containing oxidised oils or fresh oil was increased body weight, feed intake, and weight gain (p < .05). The dietary antioxidants had no effect on plasma GPx in birds fed oxidised oil. Dietary supplementation with OPLE and α-Toc increased SOD activity and reduced TBARs in the thigh meat of broilers fed with a diet containing oxidised oil (p < .05). Birds fed with the dietary oxidised oil revealed lower titres of antibody against the sheep red blood cell (p < .05), and immune response was significantly improved by the supplementary OPLE. Serum cholesterol, triacylglycerols, and HDL were not affected by the inclusion of antioxidants and the type of oil. In conclusion, Otostgia persica leaf extract combined with α-Toc could reduce the negative effects of oxidised oil in the diets of broiler.

• Highlights

• Performance, plasma oxidative status, and lipid oxidation in broilers fed with oxidised oil were decreased.

• The immune response was significantly improved by the supplementary Otostgia persica leaf extract in broilers.

• Otostgia persica leaf extract reduced TBARs in thigh meat broilers fed with diet oxidised oils

• Otostgia persica leaf extract along with vitamin E has the ability to reduce the negative effects of a diet containing oxidised oil in broilers.

摘要

本研究的目的是确定维生素 E (α-Toc) 和Otostgia persica叶提取物 (OPLE) 对饲喂氧化油的肉鸡的性能、免疫反应和氧化状态的影响。总共 350 只 1 日龄雄性肉鸡被随机分配到七种日粮处理。肉鸡饲喂含新鲜油或氧化油的日粮，不含添加剂，含氧化油的日粮添加 200 mg/kg α-Toc、100 或 200 mg/kg OPLE、200 mg/kg α-Toc+ 100 mg/kg OPLE，和200 mg/kg α-Toc + 200 OPLE mg/kg，持续六周。通过测量血浆中的超氧化物歧化酶 (SOD) 和谷胱甘肽过氧化物酶 (GPx) 活性来评估血浆氧化状态。通过硫代巴比妥酸反应性物质 (TBAR) 测量大腿肉中的脂质过氧化。在含有氧化油或新鲜油的饮食中加入抗氧化剂会增加体重、采食量和体重增加。p  < .05）。膳食抗氧化剂对饲喂氧化油的鸟类的血浆 GPx 没有影响。日粮中添加 OPLE 和 α-Toc 可提高肉鸡大腿肉中的 SOD 活性并降低 TBARs ( p  < .05)。饲喂日粮氧化油的家禽的抗绵羊红细胞抗体滴度较低 ( p  < .05)，并且补充 OPLE 显着改善了免疫反应。血清胆固醇、甘油三酯和高密度脂蛋白不受抗氧化剂和油类型的影响。总之，桃叶提取物结合α-Toc可以减少氧化油在肉鸡日粮中的负面影响。

• 强调

• 饲喂氧化油的肉鸡的性能、血浆氧化状态和脂质氧化均降低。

• 在肉鸡中添加桃叶提取物可显着改善免疫反应。

• Otostgia persica叶提取物降低了饲喂日粮氧化油的大腿肉肉鸡的 TBARs

• Otostgia persica叶提取物和维生素 E 能够减少含有氧化油的日粮对肉鸡的负面影响。