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Effects of extraction method, solvent and time on the bioactive compounds and antioxidant activity of Tetrigona apicalis Malaysian propolis
Journal of Apicultural Research ( IF 1.9 ) Pub Date : 2021-06-15 , DOI: 10.1080/00218839.2021.1930958
Wan Nurul Huda Wan Zainal 1 , Nur Amirah Adlina Mohd Azian 1 , Sitti Saleha Albar 1 , Ainin Sofea Rusli 1
Affiliation  

Propolis, a type of resin produced by honey bees, has many medicinal properties. Various extraction methods and conditions had been studied to extract the bioactive compounds from raw propolis. However, different extraction methods and extraction conditions were reported to exhibit different extraction efficiencies. Hence, in this study, different extraction methods, namely maceration, ultrasound-assisted extraction and combination of maceration and ultrasound-assisted extraction, as well as extraction solvents and extraction times, were examined to investigate the effects on the amount of total phenolic content, total flavonoid content and antioxidant activity of Tetrigona apicalis Malaysian propolis extract. Experimental results showed that combination method of maceration and ultrasound-assisted extraction is the best method for extracting the highest amount of total phenolic content with 70% aqueous ethanol as solvent and obtained at the longest extraction time (24 h of maceration and 60 min of ultrasound-assisted extraction). While for total flavonoid content, maceration exhibited the best extraction method and the highest amount of flavonoid was obtained from 80% aqueous ethanol at the lowest extraction time (8 h). Meanwhile, antioxidant activity was found to have strong correlations with phenolic and flavonoid contents. In conclusion, it is crucial to develop effective extraction method and conditions depending on the targeted compounds and desired properties to enhance the extraction efficiency.



中文翻译:

提取方法、溶剂和时间对马来西亚Tetrigona apicalis马来西亚蜂胶生物活性成分和抗氧化活性的影响

蜂胶是蜜蜂生产的一种树脂,具有许多药用价值。研究了各种提取方法和条件以从原始蜂胶中提取生物活性化合物。然而,据报道,不同的提取方法和提取条件表现出不同的提取效率。因此,在本研究中,研究了不同的提取方法,即浸渍、超声辅助提取以及浸渍和超声辅助提取的组合,以及提取溶剂和提取时间,以研究对总酚含量的影响,Tetrigona apicalis总黄酮含量及抗氧化活性马来西亚蜂胶提取物。实验结果表明,以70%乙醇水溶液为溶剂,浸渍和超声辅助提取相结合的方法是提取总酚含量最高的最佳方法,且提取时间最长(浸渍24 h,超声60 min)。 -辅助提取)。而对于总黄酮含量而言,浸渍法表现出最佳的提取方法,并且在最短的提取时间(8 小时)下从 80% 乙醇水溶液中获得了最高量的黄酮类化合物。同时,发现抗氧化活性与酚类和类黄酮含量有很强的相关性。总之,根据目标化合物和所需性质开发有效的提取方法和条件以提高提取效率至关重要。

更新日期:2021-06-15
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