Liver cancer is one of the most aggressive malignant tumors. It is significant to understand the molecular mechanism of liver cancer cells to develop new treatment plans. Studies have identified that FBP1 serves as a cancer inhibitor gene. To research the effect mechanism of FBP1 in liver cancer cells, bioinformatics analysis was performed to study its expression in liver cancer tissue. Survival analysis was also performed. Moreover, starBase database was applied to predict upstream regulatory genes of FBP1. Dual-luciferase assay was performed to testify their targeted relationship. The mRNA and protein expression levels of FBP1 in liver cancer cells were detected by qRT-PCR and western blot, respectively. Cell viability was analyzed by CCK-8 assay. The migratory and invasive abilities of cells were analyzed by Transwell assay. The apoptosis of liver cancer cells was detected by flow cytometry. The results showed that the expression of FBP1 was downregulated in liver cancer tissue and cells. FBP1 low expression was correlated with the poor prognosis of patients. miR-18a-5p could inhibit FBP1 expression. Overexpression of FBP1 could inhibit the progression of liver cancer cells and promote cell apoptosis. Overexpressing miR-18a-5p could promote the progression of liver cancer cells and inhibit cell apoptosis. However, overexpressing FBP1 simultaneously could reverse the effect. miR-18a-5p and FBP1 are expected to be candidates for liver cancer treatment.

中文翻译：

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miR-18a-5p靶向FBP1促进肝癌细胞增殖、迁移和侵袭并抑制细胞凋亡

肝癌是最具侵袭性的恶性肿瘤之一。了解肝癌细胞的分子机制对于制定新的治疗方案具有重要意义。研究发现 FBP1 是一种癌症抑制基因。为了研究FBP1在肝癌细胞中的作用机制，通过生物信息学分析其在肝癌组织中的表达情况。还进行了生存分析。此外，利用starBase数据库预测FBP1的上游调控基因。进行双荧光素酶测定以证明它们的靶向关系。分别采用qRT-PCR和Western blot检测肝癌细胞中FBP1 mRNA和蛋白表达水平。通过CCK-8测定法分析细胞活力。通过Transwell实验分析细胞的迁移和侵袭能力。流式细胞仪检测肝癌细胞凋亡情况。结果显示肝癌组织和细胞中FBP1表达下调。FBP1低表达与患者不良预后相关。miR-18a-5p可以抑制FBP1的表达。FBP1的过表达可以抑制肝癌细胞的进展并促进细胞凋亡。过表达miR-18a-5p可以促进肝癌细胞的进展并抑制细胞凋亡。然而，同时过表达 FBP1 可能会逆转这种效应。miR-18a-5p 和 FBP1 有望成为肝癌治疗的候选者。