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Hormetic effects of low-dose gamma rays in soybean seeds and seedlings: A detection technique using optical sensors
Computers and Electronics in Agriculture ( IF 8.3 ) Pub Date : 2021-06-15 , DOI: 10.1016/j.compag.2021.106251
Nielsen Moreira Oliveira , André Dantas de Medeiros , Marina de Lima Nogueira , Valter Arthur , Thiago de Araújo Mastrangelo , Clíssia Barboza da Silva

The use of radiation technology in agriculture has increased remarkably in recent years. The stimulating effect of low-dose gamma rays, known as hormesis, improves the adaptive response of living organisms to more severe challenges. Different areas of agriculture such as plant physiology, crop breeding and seed biology demand accurate methods for real-time diagnosis of the ionizing radiation effect on biological systems. However, traditional measures are still calculated from slow, laborious and destructive techniques, which depend on human vision. Here, we present two robust optical sensors based on multispectral and fluorescence imaging approaches for rapid and non-destructive detection of the hormetic effects of low-dose gamma radiation in soybean seeds and seedings. Multispectral images were acquired from seeds treated with gamma radiation (0, 12, 16 and 20 Gy), and the reflectance, autofluorescence, texture and color features were extracted from images. Next, a chlorophyll fluorescence-based technique was applied to measure the minimal level of fluorescence (F0), maximum fluorescence (FM), average chlorophyll a fluorescence and quantum yield of photosystem II (FV/FM) in seedlings grown from irradiated and non-irradiated seeds. Using a reflectance technology, the seedlings were also evaluated for anthocyanin, chlorophyll a and normalized difference vegetation index (NDVI). Results showed that multispectral and fluorescence-based techniques provide useful information on the hormetic effects of gamma rays in soybean seeds and seedlings, which are invisible to the naked eye. Our findings provide a better understanding of the link between spectral and fluorescence parameters and the hormetic effect of low-dose gamma rays. From a practical point of view, such optical sensors can provide markers for fast, non-destructive and reliable identification of the best gamma-ray doses to stimulate soybean growth parameters. However, we strongly support in-depth studies of the dose–response relationship in seeds with different initial physiological potential from different soybean genotypes.



中文翻译:

低剂量伽马射线对大豆种子和幼苗的刺激作用:一种使用光学传感器的检测技术

近年来,辐射技术在农业中的应用显着增加。低剂量伽马射线的刺激作用(称为兴奋剂)可提高生物体对更严峻挑战的适应性反应。农业的不同领域,如植物生理学、作物育种和种子生物学,需要准确的方法来实时诊断电离辐射对生物系统的影响。然而,传统的措施仍然是通过缓慢、费力和破坏性的技术计算出来的,这取决于人类的视觉。在这里,我们提出了两种基于多光谱和荧光成像方法的强大光学传感器,用于快速和无损检测低剂量伽马辐射对大豆种子和种子的刺激作用。多光谱图像是从用伽马辐射 (0, 12、16 和 20 Gy),并从图像中提取反射率、自发荧光、纹理和颜色特征。接下来,应用基于叶绿素荧光的技术来测量最小荧光水平(F0 )、最大荧光 (F M )、平均叶绿素a荧光和光系统 II 的量子产率 (F V /F M ) 在从辐照和未辐照种子生长的幼苗中。使用反射技术,还评估了幼苗的花青素、叶绿素a归一化差异植被指数(NDVI)。结果表明,多光谱和基于荧光的技术提供了有关伽马射线对大豆种子和幼苗的刺激作用的有用信息,这些信息是肉眼不可见的。我们的研究结果提供了对光谱和荧光参数与低剂量伽马射线刺激效应之间联系的更好理解。从实用的角度来看,这种光学传感器可以为快速、无损和可靠地识别最佳伽马射线剂量提供标记,以刺激大豆生长参数。然而,我们强烈支持对来自不同大豆基因型的具有不同初始生理潜力的种子的剂量反应关系进行深入研究。

更新日期:2021-06-15
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