At low temperatures (18°C), seedlings of an indica rice (Oryza sativa L.) cultivar Kasalath showed symptoms of chlorosis, although the leaves of a japonica cultivar Arroz da Terra remained green. In this study, transcripts related to the chlorophyll content of rice seedlings grown at 18°C were investigated using RNA-sequencing (RNA-Seq) data for F₂ crosses between cultivars Arroz da Terra and Kasalath, as well as their parental cultivars. Differential expression analysis revealed that gene ontology terms related to ‘photosynthesis’ were significantly enriched in lowly expressed genes at 18°C than at 25°C in Kasalath. However, the gene ontology terms related to ‘response to stress’ were significantly enriched in highly expressed genes at 18°C than at 25°C in Kasalath. When the F₂ plants were grown at 18°C, their chlorophyll contents varied. Transcripts with expression levels related to chlorophyll content were statistically selected using RNA-Seq data from 21 F₂ plants. In regression models, frequently selected genes included four photosynthetic and two stress-responsive genes. The expression values of four photosynthetic and two stress-responsive genes in high-frequency selected genes were significantly correlated with chlorophyll content not only in plants analysed using RNA-Seq but also in 95 F₂ plants.

在低温 (18°C) 下，籼稻( Oryza sativa L.) 品种 Kasalath 的幼苗表现出萎黄症状，但粳稻品种 Arroz da Terra的叶子仍保持绿色。在本研究中，使用 F _{2 的}RNA 测序 (RNA-Seq) 数据研究了与 18°C 下生长的水稻幼苗叶绿素含量相关的转录本Arroz da Terra 和 Kasalath 以及它们的亲本品种之间的杂交。差异表达分析显示，与“光合作用”相关的基因本体术语在 18°C 下的低表达基因中比在 25°C 下的 Kasalath 显着丰富。然而，与“应激反应”相关的基因本体术语在 Kasalath 的 18°C 下比在 25°C 下在高表达基因中显着丰富。当 F ₂植物在 18°C 下生长时，它们的叶绿素含量会发生变化。使用来自 21 F _{2 的}RNA-Seq 数据统计选择与叶绿素含量相关的表达水平的转录本植物。在回归模型中，经常选择的基因包括四个光合基因和两个应激反应基因。高频选择基因中四个光合基因和两个胁迫响应基因的表达值与叶绿素含量显着相关，不仅在使用 RNA-Seq 分析的植物中，而且在 95 个 F ₂植物中。