当前位置: X-MOL 学术Biol. Bull. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Antibacterial Potential of Actinomycetes from North-Western Himalayas against Pathogenic Bacteria
Biology Bulletin ( IF 0.5 ) Pub Date : 2021-06-12 , DOI: 10.1134/s1062359021030146
N. Salaria , J. Furhan

Abstract

Actinomycetes derived from unfamiliar surroundings are considered as a promising supply for novel bioactive compounds comprising a broad range of biological actions. The current study was carried out to find out the bioactive adjacent to resistant pathogenic strains. During the study, 46 strains of actinomycetes were isolated from the soil sediment sample collected from Manasbal Lake of Kashmir. During the primary screening, 14 actinomycete isolates showed an inhibition zone against MTCC pathogenic cultures, including clinical isolates of Streptomyces aureus. Out of 14 isolates, 8 showed a wide range of activity. One isolate A41 was found to be propitious for its wide spectrum of antibacterial action. Phylogenetic studies of A41 strain showed maximum resemblance with Streptomyces exfoliates MMA1033. The study was extended to standardize the fermentation conditions to enhance bioactive metabolite production. For A41, actinomycetes isolation agar (AIA) was confirmed as the best growth medium for maximum antibiotic production on the 16th day at pH 7, with an inoculum size of 2.5% (w/v) at 28°C. Peptone 1% (w/v) and sucrose 1% (w/v) were optimized as the best nitrogen and carbon sources respectively for the highest antimicrobial production. The isolates were not found to be halotolerant, exhibited the highest antibiotic production at 1.5% (w/v) of NaCl. The advance exploration could provide us extremely resourceful antibacterial agents in opposition to the clinical imperative human pathogens. To the best of our knowledge, this is the first report on an assorted assembly of Actinomycetes from freshwater lakes of Kashmir having wide spectrum antimicrobial action.



中文翻译:

喜马拉雅西北部放线菌对病原菌的抗菌潜力

摘要

来自陌生环境的放线菌被认为是具有广泛生物作用的新型生物活性化合物的有希望的供应。目前的研究是为了找出与耐药病原菌株相邻的生物活性。在研究期间,46个菌株放线菌的小号从得自克什米尔Manasbal湖收集的土壤沉积物样品中分离。在初步筛选期间,14 个放线菌分离株显示出对 MTCC 致病培养物的抑制区,包括金黄色链霉菌的临床分离株。在 14 个分离株中,8 个表现出广泛的活性。发现一种分离物 A41 因其广泛的抗菌作用而有益。A41 菌株的系统发育研究显示与链霉菌去除MMA1033 的角质。该研究扩展到标准化发酵条件,以提高生物活性代谢物的产量。对于 A41,放线菌分离琼脂 (AIA) 被确认为在第 16 天在 pH 7 条件下产生最大抗生素的最佳生长培养基,在 28°C 下接种量为 2.5% (w/v)。蛋白胨 1% (w/v) 和蔗糖 1% (w/v) 分别被优化为最佳氮源和碳源,以获得最高的抗菌产量。未发现分离株具有耐盐性,在 1.5% (w/v) NaCl 时表现出最高的抗生素产量。先进的探索可以为我们提供极富足智多谋的抗菌剂,以对抗临床必需的人类病原体。据我们所知,这是关于各种组装的第一份报告来自克什米尔淡水湖的放线菌具有广谱抗菌作用。

更新日期:2021-06-13
down
wechat
bug