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The chemical characteristics and anatomy of Caucasian fir (Abies nordmanniana subsp. nordmanniana) crown gall tumors infected by Agrobacterium tumefaciens
Wood Science and Technology ( IF 3.4 ) Pub Date : 2021-06-13 , DOI: 10.1007/s00226-021-01310-9
Emrah Peşman , Funda Erşen Bak , Mustafa Umut Konanç

In this study, chemical and anatomical properties of tumorous and normal root of Abies nordmanniana subsp. nordmanniana were compared. The amount of hemicellulose in tumorous root was identified to be 5.34% higher on average than in normal root. However, the amount of lignin was measured to be around 5.78% less. It was determined that the amount of extractive substance, 1% NaOH, cold and hot water dissolution also increased with the formation of tumors. In the study, carbohydrate analysis of samples was also made and it was found out that the amount of glucose, xylose, mannose and galactose of the roots containing tumor were 2.27%, 1.18%, 1.1%, 0.37% more than normal root, respectively. On the other hand, the crystallinity of tumorous roots was decreased in spite of the increase in the amount of cellulose with the formation of tumors. According to the elemental analysis results, it was observed that carbon formation increased with tumor formation. These results were also supported by FTIR-ATR characterization and thermal analysis results. In the anatomical comparison, it was observed that tracheid lengths, tracheid thicknesses and lumen thicknesses decreased with tumor formation. Traumatic resin canals were observed in the cross sections of the tumorous root samples. In addition, it was determined that the annual ring separation was less prominent in the cross section, and the cell diameters were smaller and their location was more dispersed.



中文翻译:

根癌农杆菌感染的高加索冷杉(Abies nordmanniana subsp. nordmanniana)冠瘿瘤的化学特征和解剖结构

在这项研究中,冷杉亚种的肿瘤根和正常根的化学和解剖学特性。诺德曼尼亚纳进行了比较。经鉴定,肿瘤根中半纤维素的含量平均比正常根高 5.34%。然而,测量到木质素的量减少了约 5.78%。确定提取物、1% NaOH、冷热水溶解的量也随着肿瘤的形成而增加。研究中还对样品进行了碳水化合物分析,发现含瘤根的葡萄糖、木糖、甘露糖和半乳糖含量分别比正常根高2.27%、1.18%、1.1%、0.37% . 另一方面,尽管随着肿瘤的形成纤维素量增加,肿瘤根部的结晶度降低。根据元素分析结果,观察到碳的形成随着肿瘤的形成而增加。这些结果也得到了 FTIR-ATR 表征和热分析结果的支持。在解剖比较中,观察到管胞长度、管胞厚度和管腔厚度随着肿瘤形成而减小。在肿瘤根样品的横截面中观察到创伤性树脂管。此外,确定横截面年轮分离不那么明显,细胞直径更小,它们的位置更分散。

更新日期:2021-06-14
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