ABSTRACT

The use of nanoparticles in the appropriate portion to increase plant growth and development is an ongoing biological process. However, its exposure in an inconsiderable proportion become stressful and plant uses diverse regulatory mechanisms such as gene expression and miRNAs regulation to attain their cellular homeostasis. This study documented the influence of MgO NPs on genes and miRNAs expression, cell morphology, chlorophyll content, and physiological changes in Ananas comosus var. bracteatus at different treatment levels. It’s found that as MgO NPs exposed to the plant's increased, the average root length, average biomass, and average leaf length significantly decreased. A low concentration of MgO NPs (1 g/mL) increased the chlorophyll content of leaves, while 2 and 4 g/mL decreased it significantly. A 4 g/mL of MgO NPs increased the expression of miR-396 and miR-398 extremely, while repressed the expression of RHS12 and XTH genes significantly. Furthermore, the number of vascular bundles of root stele decreased obviously under 4 g/mL MgO NPs. 20 nm MgO NPs may have a destructive influence on the growth and development of Ananas comosus var. bracteatus by inhibiting chlorophyll.

摘要

在适当的部分使用纳米颗粒来促进植物生长和发育是一个持续的生物过程。然而，它以微不足道的比例暴露在压力下，植物使用多种调节机制，如基因表达和 miRNA 调节来实现其细胞稳态。本研究记录了 MgO NPs 对Ananas comosus var.基因和 miRNA 表达、细胞形态、叶绿素含量和生理变化的影响。苞片在不同的治疗水平。发现随着MgO NPs暴露于植物的增加，平均根长、平均生物量和平均叶长显着降低。低浓度的 MgO NPs (1 g/mL) 会增加叶子的叶绿素含量，而 2 和 4 g/mL 会显着降低叶绿素含量。A 4克/ MgO的NP的毫升增加的miR-396和miR-398极度的表达，而抑制表达RHS12和XTH显著基因。此外，在 4 g/mL MgO NPs 下，根中柱的维管束数量明显减少。20 nm MgO NPs 可能对Ananas comosus var.的生长和发育产生破坏性影响。bracteatus通过抑制叶绿素。