Cytokinins (CKs) are involved in several developmental stages in the life-cycle of plants. The CK content in plants and their respective organs are susceptible to changes under different environmental conditions. In the current study, we profiled the CK content in the above and underground organs of three legumes (Lessertia frutescens, Mucuna pruriens and Pisum sativum) grown in soils collected from four locations (Ashburton, Bergville, Hluhluwe and Izingolweni) in KwaZulu-Natal province, South Africa. The quantified CK contents in the three legumes were categorized on the basis of their side chains (isoprenoid, aromatic and furfural) and modifications (e.g. free bases and glucosides). Legume and soil types as well as their interaction significantly influenced the concentrations of CKs. Lessertia frutescens, Mucuna pruriens and Pisum sativum had CK content that ranged from 124–653, 170–670 and 69–595 pmol/g DW, respectively. Substantial quantity (> 600 pmol/g DW) of CK were observed in plants grown in Bergville (above-ground part of Lessertia frutescens) and Izingolweni (underground part of Mucuna pruriens) soils. A total of 28 CK derivatives observed in the legumes comprised of isoprenoid (22), aromatic (5) and furfural (1) side-chain CKs. However, the 16 CK derivatives in Mucuna pruriens were isoprenoid-type based on the side-chain. Generally, a higher ratio of cis-zeatin (cZ) relative to the trans-zeatin (tZ) was evident in the above-ground part of Lessertia frutescens and Pisum sativum for the four soil treatments. In terms of functional and physiological importance of the CKs, the free bases (active form) and ribosides (translocation form) were the most abundant CK in Lessertia frutescens and Pisum sativum. However, N-glucoside, a deactivation/detoxicification product was the most dominant CK in Mucuna pruriens from Hluhluwe and Izingolweni soils. The total CKs in the underground parts of the legumes had a positive significant correlation with the total phosphorus and nitrogen content in the plant as well as the soil nitrogen. Overall, the CK profiles of the legumes were strongly influenced by the soil types.

细胞分裂素 (CKs) 涉及植物生命周期的几个发育阶段。植物及其各器官中CK含量在不同环境条件下易发生变化。在目前的研究中，我们分析了三种豆科植物（小叶小豆、黧豆豆和豌豆）的上部和下部器官中的 CK 含量。) 生长在从南非夸祖鲁-纳塔尔省的四个地点（阿什伯顿、伯格维尔、赫卢赫卢韦和伊津戈尔韦尼）收集的土壤中。三种豆类中定量的 CK 含量根据其侧链（类异戊二烯、芳香族和糠醛）和修饰（例如游离碱基和糖苷）进行分类。豆科植物和土壤类型以及它们的相互作用显着影响CKs的浓度。Lessertia frutescens、Mucuna pruriens和Pisum sativum的 CK 含量分别为 124-653、170-670 和 69-595 pmol/g DW。在生长于 Bergville（ Lessertia frutescens的地上部分）和 Izingolweni（Lessertia frutescens 的地下部分）的植物中观察到大量（> 600 pmol/g DW）的 CKMucuna pruriens ) 土壤。在豆科植物中观察到总共 28 种 CK 衍生物，包括类异戊二烯 (22)、芳香族 (5) 和糠醛 (1) 侧链 CK。然而，黧豆中的 16 个 CK 衍生物基于侧链是类异戊二烯类型。总体而言，四种土壤处理的小黄菜和豌豆地上部分的顺式玉米素（c Z）相对于反式玉米素（t Z）的比例较高。就 CK 的功能和生理重要性而言，游离碱基（活性形式）和核苷（易位形式）是Lessertia frutescens和豌豆。然而，在来自 Hluhluwe 和 Izingolweni 土壤的黧毛豆粉中， N-葡萄糖苷是一种失活/解毒产物，是最主要的 CK 。豆科植物地下部分总CKs与植株全磷、全氮含量以及土壤氮含量呈显着正相关。总体而言，豆科植物的 CK 分布受土壤类型的强烈影响。